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- BOSTER
- A01728-50ul
- 中国
- 2025年07月15日
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广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01728 |
|---|---|
| 产品名称 | Anti-WNT7A Antibody |
| 基因名 | WNT7A |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 39-40KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human WNT7A (KRPTFLKIKKPLSYRKPMD). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi / Raas – Rothschild / Schinzel phocomelia syndromes. |
| 研究类别 | 1. Dang Y, Qin Y, Tang R, Mu Y, Li G, Xia M, Chen ZJ. “Variants of the WNT7A gene in Chinese patients with müllerian duct abnormalities.” Fertil Steril, 2012 Feb. 2. Kondratov AG, Kvasha SM, Stoliar LA, Romanenko AM, Zgonnyk YM, Gordiyuk VV, Kashuba EV, Rynditch AV, Zabarovsky ER, Kashuba VI. “Alterations of the WNT7A gene in clear cell renal cell carcinomas.” PLoS One, 2012. |
| Uniprot ID | WNT7A: O00755 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大Lane 1: rat kidney tissue lysate,
Lane 2: rat liver tissue lysate,
Lane 3: mouse kidney tissue lysate,
Lane 4: mouse liver tissue lysate.
Use rabbit anti- WNT7A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for WNT7A at approximately 39-40KD. The expected band size for WNT7A is at 40KD.|Figure 2. Flow cytometry analysis of ANA-1 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 3. Flow cytometry analysis of PC-3 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Purification Of Anti Peptide Antibody
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Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
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