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- BOSTER
- A00883-3-50ul
- 中国
- 2025年07月08日
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广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A00883-3 |
|---|---|
| 产品名称 | Anti-ADRA2A Antibody |
| 基因名 | ADRA2A |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 55KD |
| 免疫原 | E.coli-derived human alpha 2a Adrenergic Receptor/ADRA2A recombinant protein (Position: M16-V465). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | The alpha-2A adrenergic receptor, also known as ADRA2A denotes the human gene encoding it. This gene is mapped to 10q25.2. Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. |
| 研究类别 | 1. Philipp, M., Brede, M. E., Hadamek, K., Gessler, M., Lohse, M. J., Hein, L. Placental alpha-2-adrenoceptors control vascular development at the interface between mother and embryo. Nature Genet. 31: 311-315, 2002.2. Rosengren, A. H., Jokubka, R., Tojjar, D., Granhall, C., Hansson, O., Li, D.-Q., Nagaraj, V., Reinbothe, T. M., Tuncel, J., Eliasson, L., Groop, L., Rorsman, P., Salehi, A., Lyssenko, V., Luthman, H., Renstrom, E. Overexpression of alpha2A-adrenergic receptors contributes to type 2 diabetes. Science 327: 217-220, 2010. |
| Uniprot ID | ADRA2A: P08913 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大Lane 1: rat pancreas tissue lysates,
Lane 2: rat small intestine tissue lysates,
Lane 3: mouse pancreas tissue lysates,
Lane 4: mouse small intestine tissue lysate.
Use rabbit anti- ADRA2A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ADRA2A at approximately 55KD. The expected band size for ADRA2A is at 49KD.|Figure 2. Flow cytometry analysis of HEL cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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文献和实验a simple ELISA method to detect human anti-antibody response.
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
standard” laboratory test to diagnose or classify a patient as having APS. This chapter discusses the clinical and laboratory theoretical and technical aspects of aCL and anti-β2GPI antibody assays.
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