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- A32267-2-50ul
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- 2025年07月10日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A32267-2 |
|---|---|
| 产品名称 | Anti-ATP5F1A Antibody |
| 基因名 | ATP5F1A |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 54KD |
| 免疫原 | E.coli-derived human ATP5F1A recombinant protein (Position: R12-Q520). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | ATP synthase F1 subunit alpha, mitochondrial is an enzyme that in humans is encoded by the ATP5F1A gene. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. |
| 研究类别 | 1. Akiyama, S., Endo, H., Inohara, N., Ohta, S., Kagawa, Y. Gene structure and cell type-specific expression of the human ATP synthase alpha subunit. Biochim. Biophys. Acta 1219: 129-140, 1994. 2. Baran, A. A., Silverman, K. A., Zeskand, J., Koratkar, R., Palmer, A., McCullen, K., Curran, W. J., Jr., Edmonston, T. B., Siracusa, L. D., Buchberg, A. M. The modifier of Min 2 (Mom2) locus: embryonic lethality of a mutation in the Atp5a1 gene suggests a novel mechanism of polyp suppression. Genome Res. 17: 566-576, 2007. 3. Godbout, R., Pandita, A., Beatty, B., Bie, W., Squire, J. A. Comparative genomic hybridization analysis of Y79 and FISH mapping indicate the amplified human mitochondrial ATP synthase alpha-subunit gene (ATP5A) maps to chromosome 18q12-q21. Cytogenet. Cell Genet. 77: 253-256, 1997. |
| Uniprot ID | ATP5F1A: P25705 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Flow Cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- ATP5F1A antibody (A32267-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat H9C2(2-1) whole cell lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- ATP5F1A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP5F1A at approximately 54KD. The expected band size for ATP5F1A is at 54KD.|Figure 2. Western blot analysis of anti- ATP5F1A antibody (A32267-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: SK-OV-3 whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: HK-60 whole cell lysates,
Lane 4: RT4 whole cell lysates,
Lane 5: Daudi whole cell lysates,
Lane 6: THP-1 whole cell lysates,
Lane 7: HepG2 whole cell lysates.
Use rabbit anti- ATP5F1A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP5F1A at approximately 54KD. The expected band size for ATP5F1A is at 54KD.|Figure 3. IHC analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human endometrial adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human-rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human thyroid cance tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 7. IF analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human colon cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).|Figure 8. IF analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human placenta tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).|Figure 9. ICC analysis using anti- ATP5F1A antibody (A32267-2). was detected in immersion fixed HepG2 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).|Figure 10. Flow cytometry analysis of U937 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: rat heart tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat H9C2(2-1) whole cell lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- ATP5F1A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP5F1A at approximately 54KD. The expected band size for ATP5F1A is at 54KD.|Figure 2. Western blot analysis of anti- ATP5F1A antibody (A32267-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: SK-OV-3 whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: HK-60 whole cell lysates,
Lane 4: RT4 whole cell lysates,
Lane 5: Daudi whole cell lysates,
Lane 6: THP-1 whole cell lysates,
Lane 7: HepG2 whole cell lysates.
Use rabbit anti- ATP5F1A 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP5F1A at approximately 54KD. The expected band size for ATP5F1A is at 54KD.|Figure 3. IHC analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human endometrial adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human-rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human thyroid cance tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 7. IF analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human colon cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).|Figure 8. IF analysis using anti- ATP5F1A antibody (A32267-2). detected in paraffin-embedded section of human placenta tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).|Figure 9. ICC analysis using anti- ATP5F1A antibody (A32267-2). was detected in immersion fixed HepG2 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).|Figure 10. Flow cytometry analysis of U937 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-ATP5F1A Antibody(A32267-2-50ul)
￥1180
