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- BOSTER
- A10550-1-50ul
- 中国
- 2025年07月10日
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广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A10550-1 |
|---|---|
| 产品名称 | Anti-SIGLEC12 Antibody |
| 基因名 | SIGLEC12 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 70KD |
| 免疫原 | E.coli-derived human SIGLEC12 recombinant protein (Position: S31-H287). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Sialic acid-binding Ig-like lectin 12, or Siglec-XII, is a protein that in humans, is encoded by the SIGLEC12 gene. Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. Angata, T., Varki, N. M., Varki, A. A second uniquely human mutation affecting sialic acid biology. J. Biol. Chem. 276: 40282-40287, 2001. 2. Foussias, G., Taylor, S. M., Yousef, G. M., Tropak, M. B., Ordon, M. H., Diamandis, E. P. Cloning and molecular characterization of two splice variants of a new putative member of the Siglec-3-like subgroup of Siglecs. Biochem. Biophys. Res. Commun. 284: 887-899, 2001. 3. Mitra, N., Banda, K., Altheide, T. K., Schaffer, L., Johnson-Pais, T. L., Beuten, J., Leach, R. J., Angata, T., Varki, N., Varki, A. SIGLEC12, a human-specific segregating (pseudo)gene, encodes a signaling molecule expressed in prostate carcinomas. J. Biol. Chem. 286: 23003-23011, 2011. |
| Uniprot ID | SIGLEC12: Q96PQ1 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大Lane 1: human HEL whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
Use rabbit anti- SIGLEC12 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SIGLEC12 at approximately 70KD. The expected band size for SIGLEC12 is at 65KD.|Figure 2. Flow cytometry analysis of HEL cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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