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- BOSTER
- A08355-1-50ul
- 中国
- 2025年07月16日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A08355-1 |
|---|---|
| 产品名称 | Anti-SLC25A24 Antibody |
| 基因名 | SLC25A24 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 45KD |
| 免疫原 | E.coli-derived human SLC25A24 recombinant protein (Position: M1-I293). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Calcium-binding mitochondrial carrier protein SCaMC-1 is a protein that in humans is encoded by the SLC25A24 gene. This gene encodes a carrier protein that transports ATP-Mg exchanging it for phosphate. Multiple transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Adolphs, N., Klein, M., Haberl, E. J., Graul-Neumann, L., Menneking, H., Hoffmeister, B. Necrotizing soft tissue infection of the scalp after fronto-facial advancement by internal distraction in a 7-year-old girl with Gorlin-Chaudhry-Moss syndrome--a case report. J. Craniomaxillofac. Surg. 39: 554-561, 2011. 2. Castori, M., Silvestri, E., Pedace, L., Marseglia, G., Tempera, A., Antigoni, I., Torricelli, F., Majore, S., Grammatico, P. Fontaine-Farriaux syndrome: a recognizable craniosynostosis syndrome with nail, skeletal, abdominal, and central nervous system anomalies. Am. J. Med. Genet. 149A: 2193-2199, 2009. 3. del Arco, A., Satrustegui, J. Identification of a novel human subfamily of mitochondrial carriers with calcium-binding domains. J. Biol. Chem. 279: 24701-24713, 2004. |
| Uniprot ID | SLC25A24: Q6NUK1 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- SLC25A24 antibody (A08355-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
Use rabbit anti- SLC25A24 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SLC25A24 at approximately 45KD. The expected band size for SLC25A24 is at 53KD.|Figure 2. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human diffuse large B cell lymphoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human duodenal papilla adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human endometrioid adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human larynx squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human lung adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human prostate adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 11. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 12. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human testicular seminoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 13. IF analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1).
SLC25A24 was detected in a paraffin-embedded section of human colon cancer tissue. FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green)(Catalog#BA1101) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 14. Flow cytometry analysis of MCF-7 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human 293T whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
Use rabbit anti- SLC25A24 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SLC25A24 at approximately 45KD. The expected band size for SLC25A24 is at 53KD.|Figure 2. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human diffuse large B cell lymphoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human duodenal papilla adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human endometrioid adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human larynx squamous cell carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human lung adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 9. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human placenta tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 10. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human prostate adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 11. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 12. IHC analysis using anti- SLC25A24 antibody (A08355-1). detected in paraffin-embedded section of human testicular seminoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 13. IF analysis of SLC25A24 using anti-SLC25A24 antibody (A08355-1).
SLC25A24 was detected in a paraffin-embedded section of human colon cancer tissue. FITC Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green)(Catalog#BA1101) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue). |Figure 14. Flow cytometry analysis of MCF-7 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SLC25A24 Antibody(A08355-1-50ul)
￥1180
