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- BOSTER
- A08783-2-50ul
- 中国
- 2025年07月07日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A08783-2 |
|---|---|
| 产品名称 | Anti-SNX4 Antibody |
| 基因名 | SNX4 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 55KD |
| 免疫原 | E.coli-derived human SNX4 recombinant protein (Position: M1-M450). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Sorting nexin-4 is a protein that in humans is encoded by the SNX4 gene. This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associated with the long isoform of the leptin receptor and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor in cell cultures, but its function is unknown. This protein may form oligomeric complexes with family members. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene. |
| 研究类别 | 1. Haft, C. R., de la Luz Sierra, M., Barr, V. A., Haft, D. H., Taylor, S. I. Identification of a family of sorting nexin molecules and characterization of their association with receptors. Molec. Cell. Biol. 18: 7278-7287, 1998. |
| Uniprot ID | SNX4: O95219 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- SNX4 antibody (A08783-2). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- SNX4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SNX4 at approximately 55KD. The expected band size for SNX4 is at 52KD.|Figure 2. IHC analysis using anti- SNX4 antibody (A08783-2). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SNX4 antibody (A08783-2). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SNX4 antibody (A08783-2). detected in paraffin-embedded section of human thyroid papillary carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SNX4 antibody (A08783-2). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.[/list_product_images]
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- SNX4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SNX4 at approximately 55KD. The expected band size for SNX4 is at 52KD.|Figure 2. IHC analysis using anti- SNX4 antibody (A08783-2). detected in paraffin-embedded section of human glioblastoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SNX4 antibody (A08783-2). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SNX4 antibody (A08783-2). detected in paraffin-embedded section of human thyroid papillary carcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SNX4 antibody (A08783-2). detected in paraffin-embedded section of human colon adenocarcinoma tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.[/list_product_images]
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Anti-SNX4 Antibody(A08783-2-50ul)
￥1180
