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- A10965-1-50ul
- 中国
- 2025年07月05日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A10965-1 |
|---|---|
| 产品名称 | Anti-SETD3 Antibody |
| 基因名 | SETD3 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 60KD |
| 免疫原 | E.coli-derived human SETD3 recombinant protein (Position: Q173-R468). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | SET domain containing 3 (SETD3) is a protein that in humans is encoded by the SETD3 gene. SETD3 is a histone methyltransferase. In mouse muscle cells, Setd3 catalyzes mono- and dimethylation of histone H3 lysine-4 and H3K36 and promotes transcription of genes required for muscle cell differentiation. |
| 研究类别 | 1. Eom, G. H., Kim, K.-B., Kim, J. H., Kim, J.-Y., Kim, J.-R., Kee, H. J., Kim, D.-W., Choe, N., Park, H.-J., Son, H.-J., Choi, S.-Y., Kook, H., Seo, S.-B. Histone methyltransferase SETD3 regulates muscle differentiation. J. Biol. Chem. 286: 34733-34742, 2011. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 2/27/2014.3. Wilkinson, A. W., Diep, J., Dai, S., Liu, S., Ooi, Y. S., Song, D., Li, T.-M., Horton, J. R., Zhang, X., Liu, C., Trivedi, D. V., Ruppel, K. M., and 10 others. SETD3 is an actin histidine methyltransferase that prevents primary dystocia. Nature 565: 372-376, 2019. |
| Uniprot ID | SETD3: Q86TU7 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- SETD3 antibody (A10965-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates,
Lane 5: human HL-60 whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human RT4 whole cell lysates.
Use rabbit anti- SETD3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SETD3 at approximately 60KD. The expected band size for SETD3 is at 67KD.|Figure 2. Western blot analysis of anti- SETD3 antibody (A10965-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat H9c2(2-1) whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse C2C12 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- SETD3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SETD3 at approximately 60KD. The expected band size for SETD3 is at 67KD.|Figure 3. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human ovarian serous cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human renal cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IF analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).|Figure 9. ICC analysis using anti- SETD3 antibody (A10965-1). was detected in immersion fixed U20S cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).|Figure 10. Flow cytometry analysis of 293T cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 11. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates,
Lane 5: human HL-60 whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human RT4 whole cell lysates.
Use rabbit anti- SETD3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SETD3 at approximately 60KD. The expected band size for SETD3 is at 67KD.|Figure 2. Western blot analysis of anti- SETD3 antibody (A10965-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat H9c2(2-1) whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse C2C12 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- SETD3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SETD3 at approximately 60KD. The expected band size for SETD3 is at 67KD.|Figure 3. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human ovarian serous cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human renal cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. IF analysis using anti- SETD3 antibody (A10965-1). detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) and counterstained with DAPI (blue).|Figure 9. ICC analysis using anti- SETD3 antibody (A10965-1). was detected in immersion fixed U20S cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).|Figure 10. Flow cytometry analysis of 293T cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 11. Flow cytometry analysis of HL-60 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SETD3 Antibody(A10965-1-50ul)
￥1180
