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- BOSTER
- A02507-1-50ul
- 中国
- 2025年07月13日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A02507-1 |
|---|---|
| 产品名称 | Anti-SGCE Antibody |
| 基因名 | SGCE |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 50KD |
| 免疫原 | E.coli-derived human SGCE recombinant protein (Position: Y38-D407). |
| 内容 | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Epsilon-sarcoglycan is a protein that in humans is encoded by the SGCE gene. This gene encodes the epsilon member of the sarcoglycan family. Sarcoglycans are transmembrane proteins that are components of the dystrophin-glycoprotein complex, which link the actin cytoskeleton to the extracellular matrix. Unlike other family members which are predominantly expressed in striated muscle, the epsilon sarcoglycan is more broadly expressed. Mutations in this gene are associated with myoclonus-dystonia syndrome. This gene is imprinted, with preferential expression from the paternal allele. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A pseudogene associated with this gene is located on chromosome 2. |
| 研究类别 | 1. Asmus, F., Salih, F., Hjermind, L. E., Ostergaard, K., Munz, M., Kuhn, A. A., Dupont, E., Kupsch, A., Gasser, T. Myoclonus-dystonia due to genomic deletions in the epsilon-sarcoglycan gene. Ann. Neurol. 58: 792-797, 2005. 2. Asmus, F., Zimprich, A., Tezenas du Montcel, S., Kabus, C., Deuschl, G., Kupsch, A., Ziemann, U., Castro, M., Kuhn, A. A., Strom, T. M., Vidailhet, M., Bhatia, K. P., Durr, A., Wood, N. W., Brice, A., Gasser, T. Myoclonus-dystonia syndrome: epsilon-sarcoglycan mutations and phenotype. Ann. Neurol. 52: 489-492, 2002. 3. DeBerardinis, R. J., Conforto, D., Russell, K., Kaplan, J., Kollros, P. R., Zackai, E. H., Emanuel, B. S. Myoclonus in a patient with a deletion of the epsilon-sarcoglycan locus on chromosome 7q21. Am. J. Med. Genet. 121A: 31-36, 2003. |
| Uniprot ID | SGCE: O43556 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| ELISA: | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- SGCE antibody (A02507-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates.
Use rabbit anti- SGCE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SGCE at approximately 50KD. The expected band size for SGCE is at 50KD.|Figure 2. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of mouse brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of rat brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of human renal cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. Flow cytometry analysis of A549 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 9. Flow cytometry analysis of PC-3 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human U251 whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates.
Use rabbit anti- SGCE 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SGCE at approximately 50KD. The expected band size for SGCE is at 50KD.|Figure 2. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of mouse brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of rat brain tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 5. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of human breast cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 6. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of human liver cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 7. IHC analysis using anti- SGCE antibody (A02507-1). detected in paraffin-embedded section of human renal cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog#SV0002) with DAB as the chromogen.|Figure 8. Flow cytometry analysis of A549 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 9. Flow cytometry analysis of PC-3 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-SGCE Antibody(A02507-1-50ul)
￥1180
