E.coli-derived human NFAT4/NF-ATc3/NFATC3 recombinant protein (Position: R32-Q880).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Nuclear factor of activated T-cells, cytoplasmic 3 is a protein that in humans is encoded by the NFATC3 gene. The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes.
研究类别
1. Hoey T, Sun YL, Williamson K, Xu X (May 1995). "Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins". Immunity. 2 (5): 461–72.2. Horsley, V., Pavlath, G. K. NFAT: ubiquitous regulator of cell differentiation and adaptation. J. Cell Biol. 156: 771-774, 2002.
[list_product_images]Figure 1. Western blot analysis of anti- NFATC3 Antibody (A02727-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: K562 whole cell lysates.
Use rabbit anti- NFATC3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NFATC3 at approximately 150KD. The expected band size for NFATC3 is at 116KD.[/list_product_images]