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- A05949-1-50ul
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- 2025年07月16日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A05949-1 |
|---|---|
| 产品名称 | Anti-RPL29 Antibody |
| 基因名 | RPL29 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 20KD |
| 免疫原 | E.coli-derived human RPL29 recombinant protein (Position: Q27-A153). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | 60S ribosomal protein L29 is a protein that in humans is encoded by the RPL29 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin. Although this gene was previously reported to map to 3q29-qter, it is believed that it is located at 3p21.3-p21.2. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| 研究类别 | 1. Garcia-Barcelo, M., Law, P. T. W., Tsui, S. K. W., Fung, K. P., Lee, C. Y., Waye, M. M. Y. Mapping of the human ribosomal large subunit protein gene RPL29 to human chromosome 3q29-qter. Genomics 46: 148-151, 1997.2. Kirn-Safran, C. B., Dayal, S., Martin-DeLeon, P. A., Carson, D. D. Cloning, expression, and chromosome mapping of the murine Hip/Rpl29 gene. Genomics 68: 210-219, 2000. 3. Law, P. T. W., Tsui, S. K. W., Lam, W. Y., Luk, S. C. W., Hwaang, D. M., Liew, C. C., Lee, C. Y., Fung, K. P., Waye, M. M. Y. A novel cDNA encoding a human homologue of ribosomal protein L29. Biochim. Biophys. Acta 1305: 105-108, 1996. |
| Uniprot ID | RPL29: P47914 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- RPL29 Antibody (A05949-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: NIH/3T3 whole cell lysates,
Lane 2: COS-7 whole cell lysates,
Lane 3: Hela whole cell lysates,
Lane 4: HepG2 whole cell lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: C6 whole cell lysates,
Lane 7: mouse pancreas tissue lysates,
Lane 8: RAW264.7 whole cell lysates.
Use rabbit anti- RPL29 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RPL29 at approximately 20KD. The expected band size for RPL29 is at 18KD.|Figure 2. IHC analysis using anti- RPL29 Antibody (A05949-1). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- RPL29 Antibody (A05949-1). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. ICC analysis using anti- RPL29 Antibody (A05949-1). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1142) and counterstained with DAPI (blue).|Figure 5. Flow cytometry analysis of HeLa cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: NIH/3T3 whole cell lysates,
Lane 2: COS-7 whole cell lysates,
Lane 3: Hela whole cell lysates,
Lane 4: HepG2 whole cell lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: C6 whole cell lysates,
Lane 7: mouse pancreas tissue lysates,
Lane 8: RAW264.7 whole cell lysates.
Use rabbit anti- RPL29 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RPL29 at approximately 20KD. The expected band size for RPL29 is at 18KD.|Figure 2. IHC analysis using anti- RPL29 Antibody (A05949-1). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- RPL29 Antibody (A05949-1). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. ICC analysis using anti- RPL29 Antibody (A05949-1). was detected in immersion fixed MCF-7 cell line. Cells were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1142) and counterstained with DAPI (blue).|Figure 5. Flow cytometry analysis of HeLa cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-RPL29 Antibody(A05949-1-50ul)
￥1180
