- ￥1180
- BOSTER
- A10628-2-50ul
- 中国
- 2025年07月05日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A10628-2 |
|---|---|
| 产品名称 | Anti-TRMT1 Antibody |
| 基因名 | TRMT1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 72KD |
| 免疫原 | E.coli-derived human TRMT1 recombinant protein (Position: A61-P626). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | This gene encodes a tRNA-modifying enzyme that acts as a dimethyltransferase, modifying a single guanine residue at position 26 of the tRNA. The encoded enzyme has both mono- and dimethylase activity when exogenously expressed, and uses S-adenosyl methionine as a methyl donor. The C-terminal region of the encoded protein has both a zinc finger motif, and an arginine/proline-rich region. Mutations in this gene have been implicated in autosomal recessive intellectual disorder (ARID). Alternative splicing results in multiple transcript variants encoding different isoforms. There is a pseudogene of this gene on the X chromosome. |
| 研究类别 | 1. Blaesius, K., Abbasi, A. A., Tahir, T. H., Tietze, A., Picker-Minh, S., Ali, G., Farooq, S., Hu, H., Latif, Z., Khan, M. N., Kaindl, A. Mutations in the tRNA methyltransferase 1 gene TRMT1 cause congenital microcephaly, isolated inferior vermian hypoplasia and cystic leukomalacia in addition to intellectual disability. Am. J. Med. Genet. 176A: 2517-2521, 2018.2. Davarniya, B., Hu, H., Kahrizi, K., Musante, L., Fattahi, Z., Hosseini, M., Maqsoud, F., Farajollahi, R., Wienker, T. F., Ropers, H. H., Najmabadi, H. The role of a novel TRMT1 gene mutation and rare GRM1 gene defect in intellectual disability in two Azeri families. PLoS One 10: e0129631, 2015. Note: Electronic Article. 3. Liu, J., Straby, K. B. The human tRNA(m(2)(2)G(26))dimethyltransferase: functional expression and characterization of a cloned hTRM1 gene. Nucleic Acids Res. 28: 3445-3451, 2000. |
| Uniprot ID | TRMT1: Q9NXH9 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- TRMT1 Antibody (A10628-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: 293T whole cell lysates.
Use rabbit anti- TRMT1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TRMT1 at approximately 72KD. The expected band size for TRMT1 is at 72KD.|Figure 2. ICC analysis using anti- TRMT1 Antibody (A10628-2). was detected in immersion fixed HELA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 3. Flow cytometry analysis of JK cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Jurkat whole cell lysates,
Lane 2: 293T whole cell lysates.
Use rabbit anti- TRMT1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for TRMT1 at approximately 72KD. The expected band size for TRMT1 is at 72KD.|Figure 2. ICC analysis using anti- TRMT1 Antibody (A10628-2). was detected in immersion fixed HELA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 3. Flow cytometry analysis of JK cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-TRMT1 Antibody(A10628-2-50ul)
￥1180
