- ￥1180
- BOSTER
- M02448-1-50ul
- 中国
- 2025年07月12日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M02448-1 |
|---|---|
| 产品名称 | Anti-DDX4 Antibody (monoclonal, 3C3) |
| 基因名 | DDX4 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:3C3） |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 79KD |
| 免疫原 | E.coli-derived human DDX4/MVH recombinant protein (Position: D3-D666). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis. |
| 研究类别 | 1. Abe, K., Noce, T. A DEAD-family protein gene, Ddx4, encoding a murine homolog of Drosophila vasa maps to the distal end of mouse chromosome 13. Mammalian Genome 8: 622-623, 1997.2. Castrillon, D. H., Quade, B. J., Wang, T. Y., Quigley, C., Crum, C. P. The human VASA gene is specifically expressed in the germ cell lineage. Proc. Nat. Acad. Sci. 97: 9585-9590, 2000. 3. Chu, D. S., Liu, H., Nix, P., Wu, T. F., Ralston, E. J., Yates, J. R., III, Meyer, B. J. Sperm chromatin proteomics identifies evolutionarily conserved fertility factors. Nature 443: 101-105, 2006. |
| Uniprot ID | DDX4: Q9NQI0 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P). |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section IHC | 1:50-400 |
| Immunofluorescence (IF) | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- DDX4 Antibody (M02448-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
Use mouse anti- DDX4 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for DDX4 at approximately 79KD. The expected band size for DDX4 is at 79KD.|Figure 2. IHC analysis using DDX4 Antibody (M02448-1). detected in paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using DDX4 Antibody (M02448-1). detected in paraffin-embedded section of rat testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4.IF analysis using anti- DDX4 Antibody (M02448-1). detected in paraffin-embedded section of mouse testis tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).|Figure 5.IF analysis using anti- DDX4 Antibody (M02448-1). detected in paraffin-embedded section of rat testis tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).[/list_product_images]
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
Use mouse anti- DDX4 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for DDX4 at approximately 79KD. The expected band size for DDX4 is at 79KD.|Figure 2. IHC analysis using DDX4 Antibody (M02448-1). detected in paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using DDX4 Antibody (M02448-1). detected in paraffin-embedded section of rat testis tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4.IF analysis using anti- DDX4 Antibody (M02448-1). detected in paraffin-embedded section of mouse testis tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).|Figure 5.IF analysis using anti- DDX4 Antibody (M02448-1). detected in paraffin-embedded section of rat testis tissues. The tissue section were stained using the Dylight488 conjugated Anti-mouse IgG Secondary Antibody ((green)(Catalog # BA1126) and counterstained with DAPI (blue).[/list_product_images]
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Anti-DDX4 Antibody (monoclonal, 3C3)(M02448-1-50ul)
￥1180
