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- BOSTER
- M06975-1-50ul
- 中国
- 2025年07月14日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M06975-1 |
|---|---|
| 产品名称 | Anti-GNG2 Antibody (monoclonal, 7C13) |
| 基因名 | GNG2 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:7C13) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 12KD |
| 免疫原 | E.coli-derived human GNG2 recombinant protein (Position: A2-D48). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 is a protein that in humans is encoded by the GNG2 gene. This gene encodes one of the gamma subunits of a guanine nucleotide-binding protein. Such proteins are involved in signaling mechanisms across membranes. Various subunits forms heterodimers which then interact with the different signal molecules. |
| 研究类别 | 1. Downes, G. B., Gilbert, D. J., Copeland, N. G., Gautam, N., Jenkins, N. A. Chromosomal mapping of five mouse G protein gamma subunits. Genomics 57: 173-176, 1999. 2. Modarressi, M. H., Taylor, K. E., Wolfe, J. Cloning, characterization, and mapping of the gene encoding the human G protein gamma-2 subunit. Biochem. Biophys. Res. Commun. 272: 610-615, 2000. 3. Wolfe, J. T., Wang, H., Howard, J., Garrison, J. C., Barrett, P. Q. T-type calcium channel regulation by specific G-protein beta-gamma subunits. Nature 424: 209-213, 2003. |
| Uniprot ID | GNG2: P59768 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- GNG2 Antibody (M06975-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
Use mouse anti- GNG2 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for GNG2 at approximately 12KD. The expected band size for GNG2 is at 8KD.|Figure 2. ICC analysis using anti- GNG2 Antibody (M06975-1). was detected in immersion fixed CACO-2 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). [/list_product_images]
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
Use mouse anti- GNG2 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for GNG2 at approximately 12KD. The expected band size for GNG2 is at 8KD.|Figure 2. ICC analysis using anti- GNG2 Antibody (M06975-1). was detected in immersion fixed CACO-2 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). [/list_product_images]
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Anti-GNG2 Antibody (monoclonal, 7C13)(M06975-1-50ul)
￥1180
