- ￥1180
- BOSTER
- M01173-1-50ul
- 中国
- 2025年07月14日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M01173-1 |
|---|---|
| 产品名称 | Anti-PKM Antibody (monoclonal, 11I4C3) |
| 基因名 | PKM |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:11I4C3) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 58KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2(475-500aa KDPVQEAWAEDVDLRVNFAMNVGKAR), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides. |
| 研究类别 | 1. Ashizawa, K., Willingham, M. C., Liang, C.-M., Cheng, S. In vivo regulation of monomer-tetramer conversion of pyruvate kinase subtype M-2 by glucose is mediated via fructose 1, 6-bisphosphate. 2. J. Biol. Chem. 266: 16842-16846, 1991.2. Christofk, H. R., Vander Heiden, M. G., Wu, N., Asara, J. M., Cantley, L. C. Pyruvate kinase M2 is a phosphotyrosine-binding protein. 3. Nature 452: 181-186, 2008. 3. Popescu, N. C., Cheng, S. Y. Chromosomal localization of the gene for a human cytosolic thyroid hormone binding protein homologous to the subunit of pyruvate kinase, subtype M(2). Somat. Cell Molec. Genet. 16: 593-598, 1990. |
| Uniprot ID | PKM: P14618 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- PKM Antibody (M01173-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane : Sh-sy5y whole cell lysates.
Use mouse anti- PKM 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PKM at approximately 58KD. The expected band size for PKM is at 58KD.|Figure 2. IHC analysis using anti- PKM Antibody (M01173-1). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- PKM Antibody (M01173-1). detected in paraffin-embedded section of Differentiated adenocarcinoma of the human rectum tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. ICC analysis using anti- PKM Antibody (M01173-1). was detected in immersion fixed A549 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 5. Flow cytometry analysis of Hela cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Hela whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane : Sh-sy5y whole cell lysates.
Use mouse anti- PKM 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for PKM at approximately 58KD. The expected band size for PKM is at 58KD.|Figure 2. IHC analysis using anti- PKM Antibody (M01173-1). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis using anti- PKM Antibody (M01173-1). detected in paraffin-embedded section of Differentiated adenocarcinoma of the human rectum tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. ICC analysis using anti- PKM Antibody (M01173-1). was detected in immersion fixed A549 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 5. Flow cytometry analysis of Hela cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-PKM Antibody (monoclonal, 11I4C3)(M01173-1-50ul)
￥1180
