- ￥1180
- BOSTER
- M00790-3-50ul
- 中国
- 2025年07月12日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M00790-3 |
|---|---|
| 产品名称 | Anti-FGG Antibody (monoclonal, 5H9) |
| 基因名 | FGG |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:5H9） |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 52KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human FGG (133-163aa IRYLQEIYNSNNQKIVNLKEKVAQLEAQCQE), different from the related mouse sequence by two amino acids, and from the related rat sequence by five amino acids. |
| 内容 | 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Fibrinogen gamma chain, also known as FGG, is a human gene found on Chromosome 4. The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms. |
| 研究类别 | 1. Budzynski, A. Z., Marder, V. J., Menache, D., Guillin, M.-C. Defect in the gamma polypeptide chain of a congenital abnormal fibrinogen (Paris I). Nature 252: 66-68, 1974.2. Ebert, R. F., Bell, W. R. Fibrinogen Baltimore III: congenital dysfibrinogenemia with a shortened gamma-subunit. Thromb. Res. 51: 251-258, 1988.3. Fornace, A. J., Jr., Cummings, D. E., Comeau, C. M., Kant, J. A., Crabtree, G. R. Structure of the human gamma-fibrinogen gene: alternate mRNA splicing near the 3-prime end of the gene produces gamma-A and gamma-B forms of gamma-fibrinogen. J. Biol. Chem. 259: 12826-12830, 1984. |
| Uniprot ID | FGG: P02679 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- FGG antibody (M00790-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEPG2 whole cell lysates,
Lane 2: human HEPG2 whole cell lysates,
Use mouse anti- FGG 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for FGG at approximately 52KD. The expected band size for FGG is at 52KD.|Figure 2. ICC analysis using anti- FGG antibody (M00790-3). was detected in immersion fixed HEP3B cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 3. Flow cytometry analysis of HEPG2 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human HEPG2 whole cell lysates,
Lane 2: human HEPG2 whole cell lysates,
Use mouse anti- FGG 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for FGG at approximately 52KD. The expected band size for FGG is at 52KD.|Figure 2. ICC analysis using anti- FGG antibody (M00790-3). was detected in immersion fixed HEP3B cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue). |Figure 3. Flow cytometry analysis of HEPG2 cell (1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-FGG Antibody (monoclonal, 5H9)(M00790-3-50ul)
￥1180
