E.coli-derived human LHCGR recombinant protein (Position: Q67-N215).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
The luteinizing hormone/choriogonadotropin receptor is a member of a subfamily of G protein-coupled receptors(GPCR) characterized by the presence of a large N-terminal extracellular domain containing several leucine-rich repeats(LRR). This glycoprotein hormone receptor family has been named the LRR-containing GPCR(LGR) family. The LHCGR gene contains 11 exons and spans approximately 80 kb and it is mapped to chromosome 2p21. In the ovary, theca, stromal, late-stage(luteinizing) granulosa, and luteal cells contain LHCGR. In the testes, Leydig cells contain LHCRG.
研究类别
1. Ascoli, M., Fanelli, F., Segaloff, D. L.The lutropin/choriogonadotropin receptor, a 2002 perspective.Endocr. Rev. 23: 141-174, 2002.2. Themmen, A. P. N., Huhtaniemi, I. T.Mutations of gonadotropins and gonadotropin receptors: elucidating the physiology and pathophysiology of pituitary-gonadal function.Endocr. Rev. 21: 551-583, 2000.
[list_product_images]Figure 1. Western blot analysis of anti- hCG receptor/LHR/LHCGR Antibody (A01120-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Use rabbit anti- LHCGR 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for LHCGR at approximately 78KD. The expected band size for LHCGR is at 79KD.[/list_product_images]