- ￥1180
- BOSTER
- A00793-1-50ul
- 中国
- 2025年07月10日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A00793-1 |
|---|---|
| 产品名称 | Anti-ERG Antibody |
| 基因名 | ERG |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 55KD |
| 免疫原 | E.coli-derived human ERG recombinant protein (Position: E20-Q274). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | ERG (ETS-related gene) is an oncogene. ERG is a member of the ETS (erythroblast transformation-specific) family of transcription factors. The ERG gene encodes for a protein, also called ERG, that functions as a transcriptional regulator. This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined. |
| 研究类别 | 1. Attard, G., Clark, J., Ambroisine, L., Fisher, G., Kovacs, G., Flohr, P., Berney, D., Foster, C. S., Fletcher, A., Gerald, W., Moller, H., Reuter, V., De Bono, J. S., Scardino, P., Cuzick, J., Cooper, C. S. Duplication of the fusion of TMPRSS2 to ERG sequences identifies fatal human prostate cancer. Oncogene 27: 253-263, 2008. 2. Bose, R., Karthaus, W. R., Armenia, J., Abida, W., Iaquinta, P. J., Zhang, Z., Wongvipat, J., Wasmuth, E. V., Shah, N., Sullivan, P. S., Doran, M. G., Wang, P., Patruno, A., Zhao, Y., International SU2C/PCF Prostate Cancer Dream Team, Zheng, D., Schultz, N., Sawyers, C. L. ERF mutations reveal a balance of ETS factors controlling prostate oncogenesis. Nature 546: 671-675, 2017. 3. Delattre, O., Zucman, J., Melot, T., Garau, X. S., Zucker, J.-M., Lenoir, G. M., Ambros, P. F., Sheer, D., Turc-Carel, C., Triche, T. J., Aurias, A., Thomas, G. The Ewing family of tumors--a subgroup of small-round-cell tumors defined by specific chimeric transcripts. New Eng. J. Med. 331: 294-299, 1994. |
| Uniprot ID | ERG: P11308 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- ERG Antibody (A00793-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: colo320 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse kidney tissue lysates.
Use rabbit anti- ERG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ERG at approximately 55KD. The expected band size for ERG is at 54KD.|Figure 2. ICC analysis using anti- ERG Antibody (A00793-1). was detected in immersion fixed A431 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). [/list_product_images]
Lane 1: colo320 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse heart tissue lysates,
Lane 4: mouse kidney tissue lysates.
Use rabbit anti- ERG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ERG at approximately 55KD. The expected band size for ERG is at 54KD.|Figure 2. ICC analysis using anti- ERG Antibody (A00793-1). was detected in immersion fixed A431 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). [/list_product_images]
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Anti-ERG Antibody(A00793-1-50ul)
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