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50ul
货号 | A03935-1 |
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产品名称 | Anti-AlaRS/AARS1 Antibody |
基因名 | AARS1 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 107KD |
免疫原 | E.coli-derived human AlaRS/AARS1 recombinant protein (Position: R729-N968). |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | An aminoacyl-tRNA synthetase (aaRS or ARS), also called tRNA-ligase, is an enzyme that attaches the appropriate amino acid onto its corresponding tRNA. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. |
研究类别 | 1. Chihade, J. W., Brown, J. R., Schimmel, P. R., Ribas de Pouplana, L. Origin of mitochondria in relation to evolutionary history of eukaryotic alanyl-tRNA synthetase. Proc. Nat. Acad. Sci. 97: 12153-12157, 2000. 2. Guo, M., Chong, Y. E., Beebe, K., Shapiro, R., Yang, X.-L., Schimmel, P. The C-Ala domain brings together editing and aminoacylation functions on one tRNA. Science 325: 744-747, 2009. Note: Erratum: Science 326: 46 only, 2009. 3. Guo, M., Chong, Y. E., Shapiro, R., Beebe, K., Yang, X.-L., Schimmel, P. Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma. Nature 462: 808-812, 2009. |
Uniprot ID | AARS1: P49588 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. |
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot (WB): | 1:500-2000 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Flow cytometry (FCM): | 1-3 μg/1x106 cells |
ELISA: | 1:100-1000 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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