E.coli-derived human SEC23B recombinant protein (Position: R190-Q581).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Protein transport protein Sec23B is a protein that in humans is encoded by the SEC23B gene. The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The function of this gene product has been implicated in cargo selection and concentration. Multiple alternatively spliced transcript variants have been identified in this gene.
研究类别
1. Bianchi, P., Fermo, E., Vercellati, C., Boschetti, C., Barcellini, W., Iurlo, A., Marcello, A. P., Righetti, P. G., Zanella, A. Congenital dyserythropoietic anemia type II (CDAII) is caused by mutations in the SEC23B gene. Hum. Mutat. 30: 1292-1298, 2009.2. Gross, M. B. Personal Communication. Baltimore, Md. 7/16/2018.3. Hamosh, A. Personal Communication. Baltimore, Md. 7/11/2018.
[list_product_images]Figure 1. Western blot analysis of anti- SEC23B antibody (A05847-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Raji whole cell lysates,Lane 2: human HEPG2 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human THP-1 whole cell lysates,Lane 6: human HL-60 whole cell lysates,Lane 7: rat liver tissue lysates,Lane 8: rat stomach tissue lysates,Lane 9: mouse liver tissue lysates,Lane 10: mouse RAW264.7 whole cell lysates.Use rabbit anti- SEC23B 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SEC23B at approximately 86KD. The expected band size for SEC23B is at 86KD.|Figure 2. Flow cytometry analysis of SiHa cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]