E.coli-derived mouse SLC44A2 recombinant protein (Position: M1-S706).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Choline transporter-like protein 2 is a protein that in humans is encoded by the SLC44A2 gene. CTL2 is a multi-pass membrane protein expressed in cells of the inner ear. CTL2 is a possible candidate for autoimmune hearing loss in humans. Present in supporting cells of the inner ear (at protein level). Only isoform 3 is expressed in inner ear vestibular tissue.
研究类别
1. Greinacher, A., Wesche, J., Hammer, E., Furll, B., Volker, U., Reil, A., Bux, J. Characterization of the human neutrophil alloantigen-3a. Nature Med. 16: 45-48, 2010. 2. O'Regan, S., Traiffort, E., Ruat, M., Cha, N., Compaore, D., Meunier, F.-M. An electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins. Proc. Nat. Acad. Sci. 97: 1835-1840, 2000.
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*稀释度需要用户自己调试,此处数据仅供参考。
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[list_product_images]Figure 1. Western blot analysis of anti- SLC44A2 antibody (A05467-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse lung tissue lysates.Use rabbit anti- SLC44A2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for SLC44A2 at approximately 80KD. The expected band size for SLC44A2 is at 80KD.|Figure 2. IHC analysis using anti- SLC44A2 antibody (A05467-1). detected in paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. Flow cytometry analysis of HEPA1-6 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]