200 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
protein G purified.
浓度
200 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Interleukin-10(IL-10 or IL10), also known as human cytokine synthesis inhibitory factor(CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al.(1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al.(1997) mapped the IL10 gene to the junction between 1q31 and 1q32.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1001) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P) .
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of IL-10 using anti- IL-10 antibody (M00021-1).
The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: recombinant mouse IL-10 protein 15ng,
Lane 2: recombinant mouse IL-10 protein 10ng.
Use rat anti- IL-10 1:1000, probed with a goat anti- rat IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1004). |Figure 2. IHC analysis of IL-10 using anti- IL-10 antibody (M00021-1).
IL-10 was detected in paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rat IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1025) with DAB as the chromogen.|Figure 3. IHC analysis of IL-10 using anti- IL-10 antibody (M00021-1).
IL-10 was detected in paraffin-embedded section of mouse lymphaden tissue. Biotinylated goat anti-rat IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1025) with DAB as the chromogen.[/list_product_images]