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- BOSTER
- A06826-1-50ul
- 中国
- 2025年07月10日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A06826-1 |
|---|---|
| 产品名称 | Anti-POGLUT1 Antibody |
| 基因名 | POGLUT1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 55KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human POGLUT1 (KTDLSNVQELLQFVKANDDVAQEIAER). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | This gene encodes a protein with both O-glucosyltransferase and O-xylosyltransferase activity which localizes to the lumen of the endoplasmic reticulum. This protein has a carboxy-terminal KTEL motif which is predicted to function as an endoplasmic reticulum retention signal. This gene is an essential regulator of Notch signalling and likely plays a role in cell fate and tissue formation during development. It may also play a role in the pathogenesis of leukemia. Mutations in this gene have been associated with the autosomal dominant genodermatosis Dowling-Degos disease 4. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. Basmanav, F. B., Oprisoreanu, A.-M., Pasternack, S. M., Thiele, H., Fritz, G., Wenzel, J., Grosser, L., Wehner, M., Wolf, S., Fagerberg, C., Bygum, A., Altmuller, J., and 9 others. Mutations in POGLUT1, encoding protein O-glucosyltransferase 1, cause autosomal-dominant Dowling-Degos disease. Am. J. Hum. Genet. 94: 135-143, 2014. 2. Chu, Q., Liu, L., Wang, W. Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner. Cell Prolif. 46: 254-262, 2013. 3. Hanneken, S., Rutten, A., Eigelshoven, S., Braun-Falco, M., Pasternack, S. M., Ruzicka, T., Nothen, M. M., Betz, R. C., Kruse, R. Morbus Galli-Galli: klinische und histopathologische Untersuchung anhand einer Fallserie von 18 Patienten. Hautarzt 62: 842-851, 2011. |
| Uniprot ID | POGLUT1: Q8NBL1 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- POGLUT1 Antibody (A06826-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HL-60 whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: U87 whole cell lysates,
Lane 4: Raji whole cell lysates,
Lane 5: Jurkat whole cell lysates,
Lane 6: Hela whole cell lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: ANA-1 whole cell lysates.
Use rabbit anti-POGLUT1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for POGLUT1 at approximately 55KD. The expected band size for POGLUT1 is at 46KD.|Figure 2. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human lymphomar tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 7. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 8. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 9. ICC analysis using anti- POGLUT1 Antibody (A06826-1). was detected in immersion fixed T47D cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).[/list_product_images]
Lane 1: HL-60 whole cell lysates,
Lane 2: HepG2 whole cell lysates,
Lane 3: U87 whole cell lysates,
Lane 4: Raji whole cell lysates,
Lane 5: Jurkat whole cell lysates,
Lane 6: Hela whole cell lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: ANA-1 whole cell lysates.
Use rabbit anti-POGLUT1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for POGLUT1 at approximately 55KD. The expected band size for POGLUT1 is at 46KD.|Figure 2. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human lymphomar tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 7. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 8. IHC analysis using anti- POGLUT1 Antibody (A06826-1). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 9. ICC analysis using anti- POGLUT1 Antibody (A06826-1). was detected in immersion fixed T47D cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).[/list_product_images]
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Anti-POGLUT1 Antibody(A06826-1-50ul)
￥1180
