A synthetic peptide corresponding to a sequence of human PPP4R1 (HQEAVEQTIMALQMDRDSDVKYFASIH).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Serine/threonine-protein phosphatase 4 regulatory subunit 1 is an enzyme that in humans is encoded by the PPP4R1 gene. This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.
研究类别
1. Kloeker, S., Wadzinski, B. E. Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. J. Biol. Chem. 274: 5339-5347, 1999.
[list_product_images]Figure 1. Western blot analysis of anti-PPP4R1 Antibody(A14144-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: COS-7 whole cell lysates.
Lane 2: K562 whole cell lysates.
Lane 3: rat brain tissue lysates,
Lane 4: rat stomach tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 4: mouse stomach tissue lysates.
Use rabbit anti-PPP4R1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PPP4R1 at approximately 130KD. The expected band size for PPP4R1 is at 107KD.|Figure 2. Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]