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- BOSTER
- A02244-50ul
- 中国
- 2025年07月11日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A02244 |
|---|---|
| 产品名称 | Anti-ARG2 Antibody |
| 基因名 | ARG2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 39KD |
| 免疫原 | E.coli-derived human ARG2 recombinant protein (Position: M1-I354). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | ARG2(arginase, type II) encodes a 355-amino acid polypeptide.?Using Northern blotting and RT-PCR,?Vockley et al.(1996)?found that?ARG2?is expressed as a 1.5-kb mRNA in a wide variety of tissues, with highest levels of expression in prostate, brain, and kidney.?By PCR analysis of somatic cell hybrid panels, fluorescence in situ hybridization, and radiation hybrid analysis,?the?ARG2 gene is mapped to 14q24.1-q24.3. ARG2?may be inducible and may be essential in the regulation of nitric oxide synthesis by modulating local arginine concentrations. Gotoh et al.(1996)?showed that?ARG2?mRNA and nitric oxide synthase(NOS) mRNA were coinduced by lipopolysaccharide in a macrophage-like cell line. Arginase II has been implicated in the regulation of the arginine/ornithine concentrations in the cell. The mitochondrial location of?ARG2?and its coinduction with ornithine aminotransferase and involvement with proline biosynthesis in lactating rat mammary gland had led to the inference that?ARG2?is involved in biosynthetic functions, as opposed to the metabolic ones of the urea cycle. |
| 研究类别 | 1. Deignan, J. L., Livesay, J. C., Yoo, P. K., Goodman, S. I., O'Brien, W. E., Iyer, R. K., Cederbaum, S. D., Grody, W. W. Ornithine deficiency in the arginase double knockout mouse. Molec. Genet. Metab. 89: 87-96, 2006.2. Gotoh, T., Sonoki, T., Nagasaki, A., Terada, K., Takiguchi, M., Mori, M. Molecular cloning of cDNA for nonhepatic mitochondrial arginase (arginase II) and comparison of its induction with nitric oxide synthase in a murine macrophage-like cell line. FEBS Lett. 395: 119-122, 1996.3. Vockley, J. G., Jenkinson, C. P., Shukla, H., Kern, R. M., Grody, W. W., Cederbaum, S. D. Cloning and characterization of the human type II arginase gene. Genomics 38: 118-123, 1996. |
| Uniprot ID | ARG2: P78540 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- ARG2 antibody (A02244).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human SW620 whole cell lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse kidney tissue lysates. Use rabbit anti- ARG2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ARG2 at approximately 39KD. The expected band size for ARG2 is at 39KD.|Figure 2. IHC analysis using anti- ARG2 antibody (A02244). detected in paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- ARG2 antibody (A02244). detected in paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. ICC analysis using anti- ARG2 antibody (A02244). was detected in immersion fixed HELA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 5. Flow cytometry analysis of HEPG2 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-ARG2 Antibody(A02244-50ul)
￥1180
