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- BOSTER
- PB9166-50ul
- 中国
- 2025年07月08日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PB9166 |
|---|---|
| 产品名称 | Anti-Caveolin-2/CAV2 Antibody |
| 基因名 | CAV2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 22KD |
| 免疫原 | E.coli-derived human Caveolin-2 recombinant protein (Position: M1-D162). Human Caveolin-2 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat Caveolin-2, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Caveolin-2 is a protein that in humans is encoded by the CAV2 gene. It is mapped to 7q31.1-q31.2. The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. Caveolin-2 is a protein related to caveolin-1 which is derived caveolin-enriched membranes. CAV2 and CAV1 are similar in most respects and they differ in their functional interactions with heterotrimeric G proteins. Both of them are expressed in neuronal cells. Caveolin-2 was upregulated in response to neuronal cell injury. |
| 研究类别 | 1. Scherer, P. E., Okamoto, T., Chun, M., Nishimoto, I., Lodish, H. F., Lisanti, M. P.Identification, sequence, and expression of caveolin-2 defines a caveolin gene family.Proc. Nat. Acad. Sci. 93: 131-135, 1996. 2. Yamazaki, K., Mizui, Y., Tanaka, I.Radiation hybrid mapping of human ADAM10 gene to chromosome 15.Genomics 45: 457-459, 1997.2. Galbiati, F., Volonte, D., Gil, O., Zanazzi, G., Salzer, J. L., Sargiacomo, M., Scherer, P. E., Engelman, J. A., Schlegel, A., Parenti, M., Okamoto, T., Lisanti, M. P.Expression of caveolin-1 and -2 in differentiating PC12 cells and dorsal root ganglion neurons: caveolin-2 is up-regulated in response to cell injury.Proc. Nat. Acad. Sci. 95: 10257-10262, 1998.3. Engelman, J. A., Zhang, X. L., Galbiati, F., Lisanti, M. P.Chromosomal localization, genomic organization, and developmental expression of the murine caveolin gene family (Cav-1, -2, and -3): Cav-1 and Cav-2 genes map to a known tumor suppressor locus (6-A2/7q31).FEBS Lett. 429: 330-336, 1998. |
| Uniprot ID | CAV2: P51636 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunohistochemistry in frozen section (IHC-F): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-CAV2 antibody (PB9166). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HT1080 whole cell lysates,Lane 2: human U2OS whole cell lysates,Lane 3: human CACO-2 whole cell lysates,Lane 4: human HELA whole cell lysates,Lane 5:human A549 whole cell lysates,Lane 6: Rat lung tissue lysates,Lane 7: Mouse lung tissue lysatesUse rabbit anti- CAV2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CAV2 at approximately 22KD. The expected band size for CAV2 is at 18KD.|Figure 2.IHC analysis using anti- CAV2 antibody (PB9166). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3.IHC analysis using anti- CAV2 antibody (PB9166). detected in paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4.ICC analysis using anti-CAV2 antibody (PB9166) was detected in immersion fixed A549 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue). |Figure 5.IF analysis using anti-CAV2 antibody (PB9166). detected in paraffin-embedded section of human human rectal cancer tissue. The tissue section were stained using the Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) and counterstained with DAPI (blue).|Figure 6.Flow cytometry analysis of A549 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Caveolin-2/CAV2 Antibody(原货号PB0108)(PB9166-50ul)
￥1180
