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- BOSTER
- PB1101-50ul
- 中国
- 2025年07月12日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PB1101 |
|---|---|
| 产品名称 | Anti-AAZIN2 Antibody |
| 基因名 | AZIN2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 50KD |
| 免疫原 | E. coli-derived human AZIN2 recombinant protein (Position: C111-Q301). Human AZIN2 shares 89.4% amino acid (aa) sequence identity with mouse AZIN2. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Antizyme inhibitor 2 (AzI2), also known as arginine decarboxylase (ADC), is an enzyme that in humans is encoded by the AZIN2 gene. The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. |
| 研究类别 | 1. Pitk nen LT, Heiskala M, Andersson LC (2001). "Expression of a novel human ornithine decarboxylase-like protein in the central nervous system and testes".Biochem. Biophys. Res. Commun. 287 (5): 1051–7.2. Zhu MY, Iyo A, Piletz JE, Regunathan S (2004). "Expression of human arginine decarboxylase, the biosynthetic enzyme for agmatine". Biochim. Biophys. Acta1670 (2): 156–64. |
| Uniprot ID | AZIN2: Q96A70 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Western blot analysis of AZIN2 expression in rat brain extract (lane 1), mouse brain extract (lane 2) and HELA whole cell lysates (lane 3). AZIN2 at 50KD was detected using rabbit anti- AZIN2 Antigen Affinity purified polyclonal antibody (Catalog # PB1101) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).|AZIN2 was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti- AZIN2 Antigen Affinity purified polyclonal antibody (Catalog # PB1101) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).|AZIN2 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti- AZIN2 Antigen Affinity purified polyclonal antibody (Catalog # PB1101) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).|Figure 4. IHC analysis of AZIN2 using anti- AZIN2 antibody (PB1101).
AZIN2 was detected in paraffin-embedded section of human testicar cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- AZIN2 Antibody (PB1101) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
AZIN2 was detected in paraffin-embedded section of human testicar cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- AZIN2 Antibody (PB1101) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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Anti-L-Arginine decarboxylase/AZIN2 Antibody(PB1101-50ul)
￥1180
