E. coli-derived human Cathepsin G recombinant protein (Position: R25-E252). Human Cathepsin G shares 69.7% amino acid (aa) sequence identity with mouse Cathepsin G.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSG gene. This gene is mapped to 14q12. In transgenic mice, it was found that human Cathepsin G gene was expressed in early myeloid precursors in a manner coordinate with the expression of the endogenous murine gene in the bone marrow and spleen. The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.
研究类别
1. "Entrez Gene: CTSG cathepsin G".2. Grisolano, J. L., Sclar, G. M., Ley, T. J. Early myeloid cell-specific expression of the human Casthepsin G gene in transgenic mice. Proc. Nat. Acad. Sci. 91: 8989-8993, 1994.
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(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*稀释度需要用户自己调试,此处数据仅供参考。
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[list_product_images]Western blot analysis of Cathepsin G expression in COLO320 whole cell lysates (lane 1), MCF-7 whole cell lysates (lane 2) and U20S whole cell lysates (lane 3). Cathepsin G at 35KD was detected using rabbit anti- Cathepsin G Antigen Affinity purified polyclonal antibody (Catalog # PB10053) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).|Cathepsin G was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- Cathepsin G Antigen Affinity purified polyclonal antibody (Catalog # PB10053) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).[/list_product_images]