- ￥1180
- BOSTER
- PB10002-50ul
- 中国
- 2025年07月11日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PB10002 |
|---|---|
| 产品名称 | Anti-HMGB2 Antibody |
| 基因名 | HMGB2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 24KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB2 (65-97aa KSDKARYDREMKNYVPPKGDKKGKKKDPNAPKR), identical to the related mouse and rat sequences. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | High-mobility group protein B2, also known as high-mobility group protein 2 (HMG-2), is a protein that in humans is encoded by the HMGB2 gene. This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. |
| 研究类别 | 1. "Entrez Gene: HMGB2 high-mobility group box 2".2. Majumdar A, Brown D, Kerby S, Rudzinski I, Polte T, Randhawa Z, Seidman MM (Dec 1991). "Sequence of human HMG2 cDNA". Nucleic Acids Research 19 (23): 6643. |
| Uniprot ID | HMGB2: P26583 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- HMGB2 antibody (PB10002). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse spleen tissue lysates.
Use rabbit anti- HMGB2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HMGB2 at approximately 24KD. The expected band size for HMGB2 is at 24KD.|Figure 2. IHC analysis using anti- HMGB2 antibody (PB10002). detected in paraffin-embedded section of mouse intestine tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- HMGB2 antibody (PB10002). detected in paraffin-embedded section of rat spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- HMGB2 antibody (PB10002). detected in paraffin-embedded section of human intestinal cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 5. IF analysis using anti- HMGB2 antibody (PB10002). detected in paraffin-embedded section of rat intestine tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).|Figure 6. ICC analysis using anti- HMGB2 antibody (PB10002). was detected in immersion fixed U20S cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127).|Figure 7. ICC analysis using anti- HMGB2 antibody (PB10002) and anti-Tubulin alpha antibody (M03989-3). were detected in immersion fixed MCF7 cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126).|Figure 8. Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse spleen tissue lysates.
Use rabbit anti- HMGB2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HMGB2 at approximately 24KD. The expected band size for HMGB2 is at 24KD.|Figure 2. IHC analysis using anti- HMGB2 antibody (PB10002). detected in paraffin-embedded section of mouse intestine tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 3. IHC analysis using anti- HMGB2 antibody (PB10002). detected in paraffin-embedded section of rat spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 4. IHC analysis using anti- HMGB2 antibody (PB10002). detected in paraffin-embedded section of human intestinal cancer tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.|Figure 5. IF analysis using anti- HMGB2 antibody (PB10002). detected in paraffin-embedded section of rat intestine tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and counterstained with DAPI (blue).|Figure 6. ICC analysis using anti- HMGB2 antibody (PB10002). was detected in immersion fixed U20S cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127).|Figure 7. ICC analysis using anti- HMGB2 antibody (PB10002) and anti-Tubulin alpha antibody (M03989-3). were detected in immersion fixed MCF7 cell line. Cells were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti- mouse IgG Secondary Antibody (green)(Catalog # BA1126).|Figure 8. Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-HMGB2 Antibody(原货号PB1054)(PB10002-50ul)
￥1180
