- ￥1180
- BOSTER
- PB0977-50ul
- 中国
- 2025年07月15日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PB0977 |
|---|---|
| 产品名称 | Anti-CD9 Antibody |
| 基因名 | CD9 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 25KD |
| 免疫原 | E. coli-derived mouse CD9 recombinant protein (Position: T110-I193). Mouse CD9 shares 77.4% and 86.9% amino acid (aa) sequence identity with human and rat CD9, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | CD9 antigen is a protein that in humans is encoded by the CD9 gene. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization. While oocytes are ovulated, CD9-deficient oocytes are not properly fused with sperm upon fertilization. CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli. |
| 研究类别 | 1. Katz F, Povey S, Parkar M, Schneider C, Sutherland R, Stanley K, Solomon E, Greaves M (March 1984). "Chromosome assignment of monoclonal antibody-defined determinants on human leukemic cells". Eur J Immunol 13(12): 1008–1013. 2. Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C (2000). "Severely Reduced Female Fertility in CD9-Deficient Mice". Science 287(5451): 319–321.3. Runge KE, Evans JE, He ZY, Gupta S, McDonald KL, Stahlberg H, Primakoff P, Myles DG (2007). "Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution".Developmental Biology 304 (1): 317–325. |
| Uniprot ID | CD9: P40240 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- CD9 antibody (PB0977). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat thymus tissue lysates,Lane 2: rat lung tissue lysates,Lane 3: mouse spleen tissue lysates,Lane 4: rat lung tissue lysates,Lane 5: mouse HEPA1-6 whole cell lysates, Use rabbit anti- CD9 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD9 at approximately 25KD. The expected band size for CD9 is at 25KD.|Figure 2.CD9 was detected in paraffin-embedded sections of mouse spleen tissues, Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- CD9 antibody (PB0977). detected in paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. ICC analysis using anti- CD9 antibody (PB0977). was detected in immersion fixed HEPA1-6 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue). |Figure 5. Flow cytometry analysis of RAW264.7 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-CD9 Antibody(PB0977-50ul)
￥1180
