- ￥1180
- BOSTER
- PB0971-50ul
- 中国
- 2025年07月09日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PB0971 |
|---|---|
| 产品名称 | Anti-CAT Antibody |
| 基因名 | CAT |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 65KD |
| 免疫原 | E. coli-derived human Catalase recombinant protein (Position: E344-L527). Human Catalase shares 85.3% and 82.6% amino acid (aa) sequence identity with mouse and rat Catalase, respectively. |
| 内容 | Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Catalase is a key antioxidant enzyme in the bodies defensing against oxidative stress. It is also a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. |
| 研究类别 | 1. Junien, C., Turleau, C., de Grouchy, J., Said, R., Rethore, M.-O., Tenconi, R., Dufier, J. L. Regional assignment of catalase (CAT) gene to band 11p13: association with the aniridia-Wilms' tumor-gonadoblastoma (WAGR) complex. Ann. Genet. 23: 165-168, 1980. 2. Ogata, M., Wang, D.-H., Ogino, K. Mammalian acatalasemia: the perspectives of bioinformatics and genetic toxicology. Acta Med. Okayama 62: 345-361, 2008. Note: Erratum: Acta Med. Okayama 63: 121-122, 2009. 3. Quan, F., Korneluk, R. G., Tropak, M. B., Gravel, R. A. Isolation and characterization of the human catalase gene. Nucleic Acids Res. 14: 5321-5335, 1986. |
| Uniprot ID | CAT: P04040 |
| 推荐配套的二抗和检测试剂 | Boster provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit EK1002 in WB, supported by SA1022 in IHC(P), IHC(F) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunohistochemistry in frozen section: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- CAT antibody (PB0971). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: A549 whole cell lysates,
Lane 2: HL-60 whole cell lysates,
Lane 3: THP-1 whole cell lysates,
Lane 4: U937 whole cell lysates,
Lane 5: HepG2 whole cell lysates,
Lane 6: K562 whole cell lysates,
Lane 7: HL-60 whole cell lysates,
Lane 8: HCCT whole cell lysates,
Lane 9: HCCP whole cell lysates.
Use rabbit anti- CAT 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CAT at approximately 65KD. The expected band size for CAT is at 60KD.|Figure 2. Western blot analysis of anti- CAT antibody (PB0971). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidey tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: RH35 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse kidey tissue lysates,
Lane 7: mouse lung tissue lysates.
Use rabbit anti- CAT 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CAT at approximately 65KD. The expected band size for CAT is at 60KD.|Figure 3. IHC analysis using anti- CAT antibody (PB0971). detected in paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- CAT antibody (PB0971). detected in paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- CAT antibody (PB0971). detected in paraffin-embedded section of human liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IF analysis of Catalase using anti- Catalase antibody (PB0971).Catalase was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- Catalase Antibody (PB0971) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 7. Flow Cytometry analysis of SiHa cells using anti- Catalase antibody (PB0971).Overlay histogram showing SiHa cells stained with PB0971 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- Catalase Antibody (PB0971,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: A549 whole cell lysates,
Lane 2: HL-60 whole cell lysates,
Lane 3: THP-1 whole cell lysates,
Lane 4: U937 whole cell lysates,
Lane 5: HepG2 whole cell lysates,
Lane 6: K562 whole cell lysates,
Lane 7: HL-60 whole cell lysates,
Lane 8: HCCT whole cell lysates,
Lane 9: HCCP whole cell lysates.
Use rabbit anti- CAT 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CAT at approximately 65KD. The expected band size for CAT is at 60KD.|Figure 2. Western blot analysis of anti- CAT antibody (PB0971). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidey tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: RH35 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse kidey tissue lysates,
Lane 7: mouse lung tissue lysates.
Use rabbit anti- CAT 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CAT at approximately 65KD. The expected band size for CAT is at 60KD.|Figure 3. IHC analysis using anti- CAT antibody (PB0971). detected in paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. IHC analysis using anti- CAT antibody (PB0971). detected in paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5. IHC analysis using anti- CAT antibody (PB0971). detected in paraffin-embedded section of human liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6. IF analysis of Catalase using anti- Catalase antibody (PB0971).Catalase was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- Catalase Antibody (PB0971) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 7. Flow Cytometry analysis of SiHa cells using anti- Catalase antibody (PB0971).Overlay histogram showing SiHa cells stained with PB0971 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- Catalase Antibody (PB0971,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-Catalase Antibody(PB0971-50ul)
￥1180
