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- BOSTER
- PB0936-50ul
- 中国
- 2025年07月11日
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- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PB0936 |
|---|---|
| 产品名称 | Anti-DIABLO Antibody |
| 基因名 | DIABLO |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 25KD |
| 免疫原 | E. coli-derived human Smac/Diablo recombinant protein (Position: A56-D239). Human Smac/Diablo shares 88.6% amino acid (aa) sequence identity with mouse Smac/Diablo. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| 研究类别 | 1. "Entrez Gene: DIABLO diablo homolog (Drosophila)".2. McNeish IA, Bell S, McKay T, Tenev T, Marani M, Lemoine NR (Jun 2003). "Expression of Smac/DIABLO in ovarian carcinoma cells induces apoptosis via a caspase-9-mediated pathway". Experimental Cell Research 286 (2): 186–98.3. Yu J, Wang P, Ming L, Wood MA, Zhang L (Jun 2007). "SMAC/Diablo mediates the proapoptotic function of PUMA by regulating PUMA-induced mitochondrial events". Oncogene 26 (29): 4189–98. |
| Uniprot ID | DIABLO: Q9NR28 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| ELISA: | 1:100-1000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Western blot analysis of Smac/Diablo expression in rat testis extract (lane 1), 22RV1 whole cell lysates (lane 2) and SKOV whole cell lysates (lane 3). Smac/Diablo at 25KD was detected using rabbit anti- Smac/Diablo Antigen Affinity purified polyclonal antibody (Catalog # PB0936) at0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).|Smac/Diablo was detected in paraffin-embedded sections of mouse testis tissues using rabbit anti- Smac/Diablo Antigen Affinity purified polyclonal antibody (Catalog # PB0936) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).|Smac/Diablo was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- Smac/Diablo Antigen Affinity purified polyclonal antibody (Catalog # PB0936) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).|Smac/Diablo was detected in paraffin-embedded sections of human testis tissues using rabbit anti- Smac/Diablo Antigen Affinity purified polyclonal antibody (Catalog # PB0936) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).|Figure 5.Immunofluorescence assay analysis of U2OS cell at a dilution of 1:200 dylight 550 goat anti-rabbit IgG(red) blue Pseudocolor blue=DAPI.|Figure 6.Flow cytometry analysis of K562 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Smac/Diablo Antibody(PB0936-50ul)
￥1180
