- ￥1180
- BOSTER
- PB0670-50ul
- 中国
- 2025年07月16日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PB0670 |
|---|---|
| 产品名称 | Anti-HSP90B/GRP94 alpha Antibody |
| 基因名 | HSP90B1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 100KD |
| 免疫原 | E.coli-derived human GRP94 recombinant protein (Position: R43-H221). Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Heat shock protein 90kDa beta member 1 (HSP90B1), known as endoplasmin, or GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. |
| 研究类别 | 1. Chen B, Piel WH, Gui L, Bruford E, Monteiro A (December 2005). "The HSP90 family of genes in the human genome: insights into their divergence and evolution". Genomics 86 (6): 627–37.2. Maki RG, Old LJ, Srivastava PK (August 1990). "Human homologue of murine tumor rejection antigen gp96: 5'-regulatory and coding regions and relationship to stress-induced proteins". Proc. Natl. Acad. Sci. U.S.A.87 (15): 5658–62.3. Randow F, Seed B (2001). "Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability.". Nat. Cell Biol. 3 (10): 891–6. |
| Uniprot ID | HSP90B1: P14625 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Anti-HSP90B1 antibody (PB0670). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: A431 whole cell lysates,
Lane 4: Caco-2 whole cell lysates,
Lane 5: THP-1 whole cell lysates,
Lane 6: Caco-2 whole cell lysates,
Lane 7: HEPG2 whole cell lysates,
Lane 8: HL-60 whole cell lysates.
Use rabbit Anti-HSP90B1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HSP90B1 at approximately 100KD. The expected band size for HSP90B1 is at 92KD.|Figure 2. Western blot analysis of Anti-HSP90B1 antibody (PB0670). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: PC-12 whole cell lysates,
Lane 2: RH35 whole cell lysates,
Lane 3: C6 whole cell lysates,
Lane 4: HEPA1-6 whole cell lysates,
Lane 5: NIH/3T3 whole cell lysates,
Lane 6: RAW264.7 whole cell lysates,
Lane 7: SP2/0 whole cell lysates,
Lane 8: ANA-1 whole cell lysates.
Use rabbit Anti-HSP90B1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HSP90B1 at approximately 100KD. The expected band size for HSP90B1 is at 92KD.|Figure 3.IHC analysis using anti- HSP90B1 antibody (PB0670). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4.IHC analysis using anti- HSP90B1 antibody (PB0670). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5.IHC analysis using anti- HSP90B1 antibody (PB0670). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6.ICC analysis using anti-HSP90B1 antibody (PB0670) was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).|Figure 7.Flow cytometry analysis of THP-1 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line). [/list_product_images]
Lane 1: HELA whole cell lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: A431 whole cell lysates,
Lane 4: Caco-2 whole cell lysates,
Lane 5: THP-1 whole cell lysates,
Lane 6: Caco-2 whole cell lysates,
Lane 7: HEPG2 whole cell lysates,
Lane 8: HL-60 whole cell lysates.
Use rabbit Anti-HSP90B1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HSP90B1 at approximately 100KD. The expected band size for HSP90B1 is at 92KD.|Figure 2. Western blot analysis of Anti-HSP90B1 antibody (PB0670). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: PC-12 whole cell lysates,
Lane 2: RH35 whole cell lysates,
Lane 3: C6 whole cell lysates,
Lane 4: HEPA1-6 whole cell lysates,
Lane 5: NIH/3T3 whole cell lysates,
Lane 6: RAW264.7 whole cell lysates,
Lane 7: SP2/0 whole cell lysates,
Lane 8: ANA-1 whole cell lysates.
Use rabbit Anti-HSP90B1 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HSP90B1 at approximately 100KD. The expected band size for HSP90B1 is at 92KD.|Figure 3.IHC analysis using anti- HSP90B1 antibody (PB0670). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4.IHC analysis using anti- HSP90B1 antibody (PB0670). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5.IHC analysis using anti- HSP90B1 antibody (PB0670). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 6.ICC analysis using anti-HSP90B1 antibody (PB0670) was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).|Figure 7.Flow cytometry analysis of THP-1 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line). [/list_product_images]
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Anti-GRP94/HSP90B1 Antibody(PB0670-50ul)
￥1180
