Anti-Smad3 Antibody(PB0445-50u

[list_product_images]Figure 1. Western blot analysis of SMAD3 using anti-SMAD3 antibody (PB0445). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: U87 Whole Cell LysateLane 2: Human Placenta Tissue LysateLane 3: PANC Whole Cell LysateLane 4: HELA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD3 antigen affinity purified polyclonal antibody (Catalog # PB0445) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD3 at approximately 56KD. The expected band size for SMAD3 is at 48KD.[/list_product_images]