- ￥1180
- BOSTER
- PB0377-50ul
- 中国
- 2025年07月11日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | PB0377 |
|---|---|
| 产品名称 | Anti-survivin/BIRC5 Antibody |
| 基因名 | BIRC5 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 16-18KD |
| 免疫原 | E.coli-derived mouse Survivin recombinant protein (Position: M1-A140). Mouse Survivin shares 85% and 91% amino acid (aa) sequence identity with human and rat Survivin, respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions as inhibitor caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells. |
| 研究类别 | 1. Ambrosini, G., Adida, C., Altieri, D. C.A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma.Nature Med. 3: 917-921, 1997.2. Chantalat, L., Skoufias, D. A., Kleman, J.-P., Jung, B., Dideberg, O., Margolis, R. L.Crystal structure of human survivin reveals a bow tie-shaped dimer with two unusual alpha-helical extensions.Molec. Cell 6: 183-189, 2000. |
| Uniprot ID | BIRC5: O70201 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of Anti-BIRC5 antibody (PB0377). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Mouse thymus tissue lysates,
Lane 2: Mouse SP2/0 whole cell lysates,
Lane 3: Mouse RAW264.7 whole cell lysates,
Lane 4: Mouse ANA-1 whole cell lysates.
Use rabbit Anti-BIRC5 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BIRC5 at approximately 16KD. The expected band size for BIRC5 is at 16KD.|Figure 2. IHC analysis using anti-BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse lymph node tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. Flow cytometry analysis of NEURO-2A cell(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: Mouse thymus tissue lysates,
Lane 2: Mouse SP2/0 whole cell lysates,
Lane 3: Mouse RAW264.7 whole cell lysates,
Lane 4: Mouse ANA-1 whole cell lysates.
Use rabbit Anti-BIRC5 1:1000, probed with a goat Anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BIRC5 at approximately 16KD. The expected band size for BIRC5 is at 16KD.|Figure 2. IHC analysis using anti-BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse testis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- BIRC5 antibody (PB0377). detected in paraffin-embedded section of mouse lymph node tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. Flow cytometry analysis of NEURO-2A cell(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-Survivin/BIRC5 Antibody(PB0377-50ul)
￥1180
