- ￥1180
- BOSTER
- M09565-50ul
- 中国
- 2025年07月09日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M09565 |
|---|---|
| 产品名称 | Anti-ATP5PD Antibody (monoclonal, 6B12) |
| 基因名 | ATP5H |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:6B12) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 22KD |
| 免疫原 | E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H,respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core,F1,and the membrane-spanning component,Fo,which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha,beta,gamma,delta,and epsilon) assembled in a ratio of 3 alpha,3 beta,and a single representative of the other 3. The Fo seems to have nine subunits (a,b,c,d,e,f,g,F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition,three pseudogenes are located on chromosomes 9,12 and 15. |
| Uniprot ID | ATP5H: O75947 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-ATP5H antibody (M09565). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: monkey COS-7 whole cell lysates,
Lane 6: human SW620 whole cell lysates.
Lane 7: rat PC-12 whole cell lysates.
Lane 8: mouse Ana-1 whole cell lysates.Use mouse anti- ATP5H 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ATP5H at approximately 22KD. The expected band size for ATP5H is at 18KD.|Figure 2. IHC analysis of anti- ATP5H antibody (M09565).detected in paraffin-embedded section of human B lymphocytic tumorT tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis of anti- ATP5H antibody (M09565).detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. ICC analysis of anti- ATP5H antibody (M09565).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1032) and counterstained with DAPI (blue).[/list_product_images]
Lane 1: human Hela tissue lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: monkey COS-7 whole cell lysates,
Lane 6: human SW620 whole cell lysates.
Lane 7: rat PC-12 whole cell lysates.
Lane 8: mouse Ana-1 whole cell lysates.Use mouse anti- ATP5H 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ATP5H at approximately 22KD. The expected band size for ATP5H is at 18KD.|Figure 2. IHC analysis of anti- ATP5H antibody (M09565).detected in paraffin-embedded section of human B lymphocytic tumorT tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3. IHC analysis of anti- ATP5H antibody (M09565).detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. ICC analysis of anti- ATP5H antibody (M09565).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1032) and counterstained with DAPI (blue).[/list_product_images]
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Anti-ATP5H Antibody (monoclonal,6B12)(M09565-50ul)
￥1180
