- ￥1180
- BOSTER
- M02372-1-50ul
- 中国
- 2025年07月10日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M02372-1 |
|---|---|
| 产品名称 | Anti-ACLY Antibody (monoclonal, 5I2) |
| 基因名 | ACLY |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:5I2) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 127KD |
| 免疫原 | E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | ATP citrate lyase, aslo known as ACLY, is an enzyme that in animals represents an important step in fatty acid biosynthesis. ATP citrate lyase is the primary enzyme responsible for the synthesis of°Cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and°Cholesterogenesis. It is activated by insulin. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites. |
| Uniprot ID | ACLY: P53396 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-ATP citrate lyase antibody (M02372-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Human placenta tissue lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: HEK293 whole cell lysates,
Lane 4: Caco-2 whole cell lysates,
Lane 5: HL-60 whole cell lysates,
Lane 6: Raji whole cell lysates,
Lane 7: THP-1 whole cell lysates,
Lane 8: PANC-1 whole cell lysates,Use mouse Anti-ATP citrate lyase 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ATP citrate lyase at approximately 127KD. The expected band size for ATP citrate lyase is at 121KD.|Figure 2. Western blot analysis of anti-ATP citrate lyase antibody (M02372-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat lung tissue lysates,
Lane 2: Rat testicular tissue lysates,
Lane 3: Rat kidney tissue lysates,
Lane 4: Rat brain tissue lysates,
Lane 5: Mouse lung tissue lysates,
Lane 6: Mouse testicular tissue lysates,
Lane 7: Mouse kidney tissue lysates,Use mouse Anti-ATP citrate lyase 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ATP citrate lyase at approximately 127KD. The expected band size for ATP citrate lyase is at 121KD.|Figure 3. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of human testis cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. Flow cytometry analysis of A549 cell(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).|Figure 8.ICC analysis using anti-ATP citrate lyase antibody (M02372-1) was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).[/list_product_images]
Lane 1: Human placenta tissue lysates,
Lane 2: U-87MG whole cell lysates,
Lane 3: HEK293 whole cell lysates,
Lane 4: Caco-2 whole cell lysates,
Lane 5: HL-60 whole cell lysates,
Lane 6: Raji whole cell lysates,
Lane 7: THP-1 whole cell lysates,
Lane 8: PANC-1 whole cell lysates,Use mouse Anti-ATP citrate lyase 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ATP citrate lyase at approximately 127KD. The expected band size for ATP citrate lyase is at 121KD.|Figure 2. Western blot analysis of anti-ATP citrate lyase antibody (M02372-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat lung tissue lysates,
Lane 2: Rat testicular tissue lysates,
Lane 3: Rat kidney tissue lysates,
Lane 4: Rat brain tissue lysates,
Lane 5: Mouse lung tissue lysates,
Lane 6: Mouse testicular tissue lysates,
Lane 7: Mouse kidney tissue lysates,Use mouse Anti-ATP citrate lyase 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for ATP citrate lyase at approximately 127KD. The expected band size for ATP citrate lyase is at 121KD.|Figure 3. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 4. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of human testis cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 5. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 6. IHC analysis using Anti-ATP citrate lyase antibody (M02372-1) detected in paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 7. Flow cytometry analysis of A549 cell(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).|Figure 8.ICC analysis using anti-ATP citrate lyase antibody (M02372-1) was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).[/list_product_images]
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Anti-ATP citrate lyase Antibody (monoclonal, 5I2)(M02372-1-50ul)
￥1180
