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- BOSTER
- M01888-1-50ul
- 中国
- 2025年07月10日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M01888-1 |
|---|---|
| 产品名称 | Anti-SMARCA2 Antibody(monoclonal, 4E8) |
| 基因名 | SMARCA2 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:4E8) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 210KD |
| 免疫原 | E.coli-derived human SMARCA2/BRM recombinant protein (Position: Q181-N624). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Probable global transcription activator SNF2L2 is a protein that in humans is encoded by the SMARCA2 gene. It is mapped to 9p24.3. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. |
| 研究类别 | 1. de la Serna, I. L., Carlson, K. A., Imbalzano, A. N. Mammalian SWI/SNF complexes promote MyoD-mediated muscle differentiation. Nature Genet. 27: 187-190, 2001. 2. Hakimi, M.-A., Bochar, D. A., Schmiesing, J. A., Dong, Y., Barak, O. G., Speicher, D. W., Yokomori, K., Shiekhattar, R. A chromatin remodelling complex that loads cohesin onto human chromosomes. Nature 418: 994-998, 2002. |
| Uniprot ID | SMARCA2: P51531 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti-SMARCA2 antibody (M01888-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human U-87MG whole cell lysates.Use mouse anti- SMARCA2 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for SMARCA2 at approximately 210KD. The expected band size for SMARCA2 is at 181KD.|Figure 2.ICC analysis using anti-SMARCA2 antibody (M01888-1) was detected in immersion fixed A549 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) and counterstained with DAPI (blue).[/list_product_images]
Lane 1: human Hela whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human U-87MG whole cell lysates.Use mouse anti- SMARCA2 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for SMARCA2 at approximately 210KD. The expected band size for SMARCA2 is at 181KD.|Figure 2.ICC analysis using anti-SMARCA2 antibody (M01888-1) was detected in immersion fixed A549 cell line . Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) and counterstained with DAPI (blue).[/list_product_images]
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Anti-SMARCA2/BRM Antibody (monoclonal, 4E8)(M01888-1-50ul)
￥1180
