- ￥1180
- BOSTER
- M01847-1-50ul
- 中国
- 2025年07月11日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | M01847-1 |
|---|---|
| 产品名称 | Anti-PRDX6 Antibody(monoclonal, 6I8) |
| 基因名 | PRDX6 |
| 抗体来源 | Mouse |
| 克隆 | Monoclonal(Clone:6I8) |
| 抗体亚型 | Mouse IgG2b |
| 分子量 | 25KD |
| 免疫原 | E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224). Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6,respectively. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | protein G purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | PRDX6 is also known as PRX,p29 or AOP2. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic,fatty acid,and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. |
| Uniprot ID | PRDX6: P30041 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- PRDX6 antibody (M01847-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela tissue lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human SW620 whole cell lysates.
Lane 6: rat RH35 whole cell lysates.Use mouse anti- PRDX6 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001).A specific band was detected for PRDX6 at approximately 29KD.The expected band size for PRDX6 is at 25KD.|Figure 2. ICC analysis of anti- PRDX6 antibody (M01847-1).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1032)and counterstained with DAPI (blue).|Figure 3.Flow cytometry analysis of Hela cell(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight488. Unlabelled sample (Red line).|Figure 4. IHC analysis using anti- PRDX6 antibody (M01847-1). detected in paraffin-embedded section of human endometrial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]
Lane 1: human Hela tissue lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human SW620 whole cell lysates.
Lane 6: rat RH35 whole cell lysates.Use mouse anti- PRDX6 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001).A specific band was detected for PRDX6 at approximately 29KD.The expected band size for PRDX6 is at 25KD.|Figure 2. ICC analysis of anti- PRDX6 antibody (M01847-1).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog # BA1032)and counterstained with DAPI (blue).|Figure 3.Flow cytometry analysis of Hela cell(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight488. Unlabelled sample (Red line).|Figure 4. IHC analysis using anti- PRDX6 antibody (M01847-1). detected in paraffin-embedded section of human endometrial carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]
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Anti-peroxiredoxin 6 Antibody (monoclonal,6I8)(M01847-1-50ul)
￥1180
