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- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
50ul
产品概况
| 货号 | BM4212 |
|---|---|
| 产品名称 | Anti-CD9 Antibody |
| 基因名 | CD9 |
| 抗体来源 | Rabbit |
| 克隆 | Monoclonal |
| 抗体亚型 | Rabbit |
| 分子量 | 25KD |
| 免疫原 | A synthesized peptide derived from human CD9 |
| 内容 | 500 ug/ml;Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 纯化方式 | Affinity-chromatography |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | CD9 antigen is a protein that in humans is encoded by the CD9 gene. CD9 is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found on the surface of exosomes. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. This protein also seems to be a key part in the egg-sperm fusion during mammalian fertilization. While oocytes are ovulated, CD9-deficient oocytes are not properly fused with sperm upon fertilization. CD9 is located in the microvillar membrane of the oocytes and also appears to intervene in maintaining the normal shape of oocyte microvilli. |
| 研究类别 | 1. Katz F, Povey S, Parkar M, Schneider C, Sutherland R, Stanley K, Solomon E, Greaves M (March 1984). "Chromosome assignment of monoclonal antibody-defined determinants on human leukemic cells". Eur J Immunol 13(12): 1008–1013. 2. Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C (2000). "Severely Reduced Female Fertility in CD9-Deficient Mice". Science 287(5451): 319–321.3. Runge KE, Evans JE, He ZY, Gupta S, McDonald KL, Stahlberg H, Primakoff P, Myles DG (2007). "Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution".Developmental Biology 304 (1): 317–325. |
| Uniprot ID | CD9: P21926 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:20-100 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:20-100 |
| Immunocoprecipitation: | 1:20 |
| Flow cytometry (FCM): | 1:20 |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Western blot analysis of CD9 expression in (1)HACAT cell lysate ;(2)HepG2 cell lysate.|Immunohistochemical analysis of paraffin-embedded human tonsil, using CD9 Antibody.|Immunofluorescent analysis of Hela cells, using CD9 Antibody .[/list_product_images]
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Anti-CD9 Rabbit Monoclonal Antibody(BM4212-50ul)
¥1180







