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- BOSTER
- A05725-1-50ul
- 中国
- 2025年07月05日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A05725-1 |
|---|---|
| 产品名称 | Anti-LPAR6 Antibody |
| 基因名 | LPAR6 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 39D |
| 免疫原 | A synthetic peptide corresponding to a sequence of human P2RY5 (DTIQNSIKMKNWSVRRSDFRFSEVHGAENFIQHNLQTLK). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Lysophosphatidic acid receptor 6 also known as LPA6, P2RY5, and GPR87, is a protein that in humans is encoded by the LPAR6 gene. The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. This gene aligns with an internal intron of the retinoblastoma susceptibility gene in the reverse orientation. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. Nahum, S., Morice-Picard, F., Taieb, A., Sprecher, E. A novel mutation in LPAR6 causes autosomal recessive hypotrichosis of the scalp. Clin. Exp. Derm. 36: 188-194, 2010.2. Shimomura, Y., Wajid, M., Ishii, Y., Shapiro, L., Petukhova, L., Gordon, D., Christiano, A. M.Disruption of P2RY5, an orphan G protein-coupled receptor, underlies autosomal recessive woolly hair. Nature Genet. 40: 335-339, 2008. |
| Uniprot ID | LPAR6: P43657 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot (WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- LPAR6 antibody (A05725-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: human A549 whole cell lysates.
Use rabbit anti- LPAR6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for LPAR6 at approximately 39KD. The expected band size for LPAR6 is at 39KD.|Figure 2. Flow Cytometry analysis of A549 cells using anti-P2RY5 antibody (A05725-1).
Overlay histogram showing A549 cells stained with A05725-1 (Blue line).anti- P2RY5 Antibody (A05725-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. Flow Cytometry analysis of PC-3 cells using anti-P2RY5 antibody (A05725-1).
Overlay histogram showing PC-3 cells stained with A05725-1 (Blue line).anti- P2RY5 Antibody (A05725-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: human A549 whole cell lysates.
Use rabbit anti- LPAR6 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for LPAR6 at approximately 39KD. The expected band size for LPAR6 is at 39KD.|Figure 2. Flow Cytometry analysis of A549 cells using anti-P2RY5 antibody (A05725-1).
Overlay histogram showing A549 cells stained with A05725-1 (Blue line).anti- P2RY5 Antibody (A05725-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. Flow Cytometry analysis of PC-3 cells using anti-P2RY5 antibody (A05725-1).
Overlay histogram showing PC-3 cells stained with A05725-1 (Blue line).anti- P2RY5 Antibody (A05725-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
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Anti-P2RY5/LPAR6 Antibody(A05725-1-50ul)
￥1180
