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- BOSTER
- A05519-1-50ul
- 中国
- 2025年07月08日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A05519-1 |
|---|---|
| 产品名称 | Anti-SMOX Antibody |
| 基因名 | Smox |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 62KD |
| 免疫原 | E.coli-derived mouse SMOX recombinant protein (Position: A40-P555). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Spermine oxidase is an enzyme that in humans is encoded by the SMOX gene. It is mapped to 2; 2 F1. Polyamines are ubiquitous polycationic alkylamines which include spermine, spermidine, putrescine, and agmatine. These molecules participate in a broad range of cellular functions which include cell cycle modulation, scavenging reactive oxygen species, and the control of gene expression. These molecules also play important roles in neurotransmission through their regulation of cell-surface receptor activity, involvement in intracellular signalling pathways, and their putative roles as neurotransmitters. This gene encodes an FAD-containing enzyme that catalyzes the oxidation of spermine to spermadine and secondarily produces hydrogen peroxide. Multiple transcript variants encoding different isoenzymes have been identified for this gene, some of which have failed to demonstrate significant oxidase activity on natural polyamine substrates. The characterized isoenzymes have distinctive biochemical characteristics and substrate specificities, suggesting the existence of additional levels of complexity in polyamine catabolism. |
| 研究类别 | 1. Hartz, P. A. Personal Communication. Baltimore, Md. 6/20/2014.2. Murray-Stewart, T., Wang, Y., Devereux, W., Casero, R. A., Jr. Cloning and characterization of multiple human polyamine oxidase splice variants that code for isoenzymes with different biochemical characteristics. Biochem. J. 368: 673-677, 2002. |
| Uniprot ID | Smox: Q99K82 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of SMOX using anti-SMOX antibody (A05519-1).
Lane 1: human A549 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U2OS whole cell lysates.
anti-SMOX antigen affinity purified polyclonal antibody (Catalog # A05519-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SMOX at approximately 62KD. The expected band size for SMOX is at 62KD.|Figure 2. Western blot analysis of SMOX using anti-SMOX antibody (A05519-1).
Lane 1: rat brain issue lysates,
Lane 2: rat heart issue lysates,
Lane 3: mouse brain issue lysates,
Lane 4: mouse NIH3T3 whole cell lysates.
anti-SMOX antigen affinity purified polyclonal antibody (Catalog # A05519-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SMOX at approximately 62KD. The expected band size for SMOX is at 62KD.[/list_product_images]
Lane 1: human A549 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U2OS whole cell lysates.
anti-SMOX antigen affinity purified polyclonal antibody (Catalog # A05519-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SMOX at approximately 62KD. The expected band size for SMOX is at 62KD.|Figure 2. Western blot analysis of SMOX using anti-SMOX antibody (A05519-1).
Lane 1: rat brain issue lysates,
Lane 2: rat heart issue lysates,
Lane 3: mouse brain issue lysates,
Lane 4: mouse NIH3T3 whole cell lysates.
anti-SMOX antigen affinity purified polyclonal antibody (Catalog # A05519-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SMOX at approximately 62KD. The expected band size for SMOX is at 62KD.[/list_product_images]
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Anti-SMOX Antibody(A05519-1-50ul)
￥1180
