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- BOSTER
- A04255-50ul
- 中国
- 2025年07月10日
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A04255 |
|---|---|
| 产品名称 | Anti-CALB2 Antibody |
| 基因名 | CALB2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 32KD |
| 免疫原 | E.coli-derived human Calretinin/CALB2 recombinant protein (Position: S59-Q90). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Calretinin, also known as calbindin 2 (formerly 29 kDa calbindin), is a calcium-binding protein involved in calcium signaling. In humans, the calretinin protein is encoded by the CALB2 gene. It is mapped to 16q22.2. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. |
| 研究类别 | 1. Chen, L. Z., Harris, P. C., Apostolou, S., Baker, E., Holman, K., Lane, S. A., Nancarrow, J. K., Whitmore, S. A., Stallings, R. L., Hildebrand, C. E., Richards, R. I., Sutherland, G. R., Callen, D. F. A refined physical map of the long arm of human chromosome 16. Genomics 10: 308-312, 1991.2. De Marco Garcia, N. V., Karayannis, T., Fishell, G. Neuronal activity is required for the development of specific cortical interneuron subtypes. Nature 472: 351-355, 2011.3. Parmentier, M., Passage, E., Vassart, G., Mattei, M.-G. The human calbindin D28k (CALB1) and calretinin (CALB2) genes are located at 8q21.3-q22.1 and 16q22-q23, respectively, suggesting a common duplication with the carbonic anhydrase isozyme loci. Cytogenet. Cell Genet. 57: 41-43, 1991. |
| Uniprot ID | CALB2: P22676 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. IHC analysis using anti- CALB2 antibody (A04255). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 2. IHC analysis using anti- CALB2 antibody (A04255). detected in paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3. IHC analysis using anti- CALB2 antibody (A04255). detected in paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4. Flow cytometry analysis of C6 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 5. Flow cytometry analysis of A431 cell (1x106) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).|Figure 6. Western blot analysis of anti- CALB2 antibody (A04255). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.Use rabbit anti- CALB2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CALB2 at approximately 32KD. The expected band size for CALB2 is at 32KD.|Figure 7. ICC analysis using anti- CALB2 antibody (A04255). was detected in immersion fixed A431 cell line. Use rabbit anti- TAP2 1:50, Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) was used as secondary antibody at 1:500, The section was counterstained with DAPI (blue). [/list_product_images]
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Anti-Calretinin/CALB2 Antibody(A04255-50ul)
￥1180
