- ￥1180
- BOSTER
- A02995-2-50ul
- 中国
- 2025年06月27日
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- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A02995-2 |
|---|---|
| 产品名称 | Anti-PLSCR1 Antibody |
| 基因名 | PLSCR1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 35KD |
| 免疫原 | E.coli-derived human Scramblase 1/PLSCR1 recombinant protein (Position: M1-S54). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Phospholipid scramblase 1 is a protein that in human is encoded by the PLSCR1 gene. This gene is mapped to 3q24. |
| Uniprot ID | PLSCR1: O15162 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of anti- PLSCR1 antibody (A02995-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Caco-2 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: A431 whole cell lysates,
Lane 4: 293T whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: Raw264.7 whole cell lysates.
Use rabbit anti- PLSCR1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PLSCR1 at approximately 35KD. The expected band size for PLSCR1 is at 35KD.|Figure 2. IHC analysis of anti-PLSCR1 antibody (A02995-2).PLSCR1 was detected in paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
Lane 1: Caco-2 whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: A431 whole cell lysates,
Lane 4: 293T whole cell lysates,
Lane 5: mouse lung tissue lysates,
Lane 6: Raw264.7 whole cell lysates.
Use rabbit anti- PLSCR1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for PLSCR1 at approximately 35KD. The expected band size for PLSCR1 is at 35KD.|Figure 2. IHC analysis of anti-PLSCR1 antibody (A02995-2).PLSCR1 was detected in paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.[/list_product_images]
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Anti-Scramblase 1/PLSCR1 Antibody(A02995-2-50ul)
￥1180
