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- BOSTER
- A01586-1-50ul
- 中国
- 2025年07月10日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01586-1 |
|---|---|
| 产品名称 | Anti-NCF1 Antibody |
| 基因名 | NCF1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 47KD |
| 免疫原 | E. coli-derived human NCF1 recombinant protein (Position: M1-D270). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing. |
| 背景资料 | Neutrophil cytosol factor 1, also known as p47phox, is a protein that in humans is encoded by the NCF1 gene. The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. |
| 研究类别 | 1. Del Campo, M., Antonell, A., Magano, L. F., Munoz, F. J., Flores, R., Bayes, M., Perez Jurado, L. A. Hemizygosity at the NCF1 gene in patients with Williams-Beuren syndrome decreases their risk of hypertension. Am. J. Hum. Genet. 78: 533-542, 2006. 2. Hultqvist, M., Olofsson, P., Holmberg, J., Backstrom, B. T., Tordsson, J., Holmdahl, R. Enhanced autoimmunity, arthritis, and encephalomyelitis in mice with a reduced oxidative burst due to a mutation in the Ncf1 gene. Proc. Nat. Acad. Sci. 101: 12646-12651, 2004. |
| Uniprot ID | NCF1: P14598 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| (ELISA)： | 1:100-1000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of NCF1 using anti-NCF1 antibody (A01586-1). Lane 1: human Hela whole cell lysates,Lane 2: human blood lysates,Lane 3: rat thymus tissue lysates,Lane 4: rat lung tissue lysates,Lane 5: mouse NIH3T3 whole cell lysates. anti-NCF1 antigen affinity purified polyclonal antibody (Catalog # A01586-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for NCF1 at approximately 47KDThe expected band size for NCF1 is at 44KD.[/list_product_images]
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Anti-NCF1 Antibody(A01586-1-50ul)
￥1180
