Anti-SMC6L1 Antibody(A01554-1-

[list_product_images]Figure 1. Western blot analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human PANC-1 whole cell lysates,Lane 4: human SK-OV-3 whole cell lysates,Lane 5: human COLO-320 whole cell lysates,Lane 6: rat testis tissue lysates,Lane 7: mouse testis tissue lysates. anti-SMC6L1 antigen affinity purified polyclonal antibody (Catalog # A01554-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SMC6L1 at approximately 126KDThe expected band size for SMC6L1 is at 126KD.|Figure 2. IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1).SMC6L1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 3. IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1).SMC6L1 was detected in paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 4. IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1).SMC6L1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. |Figure 5. Flow Cytometry analysis of A431 cells using anti- SMC6L1 antibody (A01554-1).Overlay histogram showing A431 cells stained with A01554-1 (Blue line).anti- SMC6L1 Antibody A01554-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 6. IF analysis of SMC6L1 using anti- SMC6L1 antibody (A01554-1).
SMC6L1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- SMC6L1 Antibody (A01554-1) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.[/list_product_images]