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- 详细信息
- 技术资料
- 库存:
大量
- 供应商:
广州威佳科技有限公司
- 规格:
50ul
产品概况
| 货号 | A01461-1 |
|---|---|
| 产品名称 | Anti-BTG2 Antibody |
| 基因名 | BTG2 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 22KD |
| 免疫原 | A synthetic peptide corresponding to a sequence of human BTG2 (EQRLKVFSGALQEALTEHYKHHWFPEK). |
| 内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Protein BTG2, also known as BTG family member 2 or NGF-inducible anti-proliferative protein PC3 or NGF-inducible protein TIS21, is a protein that in humans is encoded by the BTG2 gene. The protein encoded by the gene BTG2 is a member of the BTG/Tob family. This protein controls cell cycle progression and proneural genes expression by acting as a transcription coregulator that enhances or inhibits the activity of transcription factors. |
| 研究类别 | 1. "Entrez Gene: BTG2 BTG family, member 2".2. Rouault JP, Falette N, Guéhenneux F, Guillot C, Rimokh R, Wang Q, Berthet C, Moyret-Lalle C, Savatier P, Pain B, Shaw P, Berger R, Samarut J, Magaud JP, Ozturk M, Samarut C, Puisieux A (December 1996). "Identification of BTG2, an antiproliferative p53-dependent component of the DNA damage cellular response pathway". Nature Genetics. 14 (4): 482–6. |
| Uniprot ID | BTG2: P78543 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of anti-BTG2 antibody (A01461-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HEPG2 Whole Cell Lysate,
Lane 2: PC-3 Whole Cell Lysate,
Lane 3: U2OS Whole Cell Lysate,
Lane 4: A549 Whole Cell Lysate.
Use rabbit anti-BTG2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BTG2 at approximately 22KD. The expected band size for BTG2 is at 17KD.|Figure 2. Flow cytometry analysis of A431 cell(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
Lane 1: HEPG2 Whole Cell Lysate,
Lane 2: PC-3 Whole Cell Lysate,
Lane 3: U2OS Whole Cell Lysate,
Lane 4: A549 Whole Cell Lysate.
Use rabbit anti-BTG2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for BTG2 at approximately 22KD. The expected band size for BTG2 is at 17KD.|Figure 2. Flow cytometry analysis of A431 cell(1x106) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-BTG2 Antibody(A01461-1-50ul)
¥1180







