Anti-Nicotinic Acetylcholine R

[list_product_images]Figure 1. Western blot analysis of CHRNA3 using anti-CHRNA3 antibody (A01981-1). Lane 1: human Hela whole cell lysates,Lane 2: human MDA-MB-453 whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: human SK-OV-3 whole cell lysates,Lane 6: human PANC-1 whole cell lysates,Lane 7: mouse thymus tissue lysates. anti-CHRNA3 antigen affinity purified polyclonal antibody (Catalog # A01981-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for CHRNA3 at approximately 60KD. The expected band size for CHRNA3 is at 57KD.|Figure 2. Flow Cytometry analysis of U251 cells using anti-CHRNA3 antibody (A01981-1).Overlay histogram showing U251 cells stained with A01981-1 (Blue line).anti-CHRNA3 Antibody (A01981-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.|Figure 3. Flow Cytometry analysis of U-87 cells using anti-CHRNA3 antibody (A01981-1).Overlay histogram showing U-87 cells stained with A01981-1 (Blue line).anti-CHRNA3 Antibody (A01981-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]