- ￥1180
- BOSTER
- A01932-2-50ul
- 中国
- 2025年07月13日
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- 库存：
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- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01932-2 |
|---|---|
| 产品名称 | Anti-FIS1 Antibody |
| 基因名 | FIS1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 17KD |
| 免疫原 | E.coli-derived human TTC11/FIS1 recombinant protein (Position: M1-D121). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Mitochondrial fission 1 protein (FIS1) is a protein that in humans is encoded by the FIS1 gene on chromosome 7. It is mapped to 7q22.1. The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. Its role in mitochondrial fission thus implicates it in the regulation of mitochondrial morphology, the cell cycle, and apoptosis. By extension, the protein is involved in associated diseases, including neurodegenerative diseases and cancers. |
| 研究类别 | 1. Dohm, J. A., Lee, S. J., Hardwick, J. M., Hill, R. B., Gittis, A. G. Cytosolic domain of the human mitochondrial fission protein Fis1 adopts a TPR fold. Proteins 54: 153-156, 2004.2. Frieden, M., James, D., Castelbou, C., Danckaert, A., Martinou, J.-C., Demaurex, N. Ca(2+) homeostasis during mitochondrial fragmentation and perinuclear clustering induced by hFis1. J. Biol. Chem. 279: 22704-22714, 2004. 3. James, D. I., Parone, P. A., Mattenberger, Y., Martinou, J.-C. hFis1, a novel component of the mammalian mitochondrial fission machinery. J. Biol. Chem. 278: 36373-36379, 2003. Note: Erratum: J. Biol. Chem. 279: 36166 only, 2004. |
| Uniprot ID | FIS1: Q9Y3D6 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC. |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry(Paraffin-embedded Section): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- FIS1 antibody (A01932-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat brain tissue lysates,Lane 2: Rat spleen tissue lysates,Lane 3: Rat kidney tissue lysates,Lane 4: Mouse brain tissue lysates,Lane 5: Mouse spleen tissue lysates,Lane 6: Mouse kidney tissue lysates,Lane 7: Mouse NIH/3T3 whole cell lysates,Lane 8: Mouse RAW264.7 whole cell lysates,Lane 9: Human HEK293 whole cell lysates,Lane 10: Human A549 whole cell lysates,Lane 11: Human HELA whole cell lysates,Lane 12: Human Raji whole cell lysates,Lane 13: Human CACO-2 whole cell lysates,Use rabbit anti- FIS1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.|Figure 2.IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 3.IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 4.IHC analysis using anti-FIS1 antibody (A01932-2). detected in paraffin-embedded section of human Ovarian cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.|Figure 5.ICC analysis using anti-FIS1 antibody (A01932-2) was detected in immersion fixed HELA cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog?#?BA1127) and counterstained with DAPI (blue). |Figure 6.Flow cytometry analysis of A549 cell(1x106) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).[/list_product_images]
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Anti-TTC11/FIS1 Antibody(A01932-2-50ul)
￥1180
