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- BOSTER
- A01767-50ul
- 中国
- 2025年07月12日
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- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01767 |
|---|---|
| 产品名称 | Anti-CD133 Antibody |
| 基因名 | PROM1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 115-130KD |
| 免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human PROM1 (808-841aa ALIFAVKLAKYYRRMDSEDVYDDVETIPMKNMEN), different from the related mouse sequence by six amino acids. |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | PROM1(Prominin 1), also called CD133, is a glycoprotein. Maw et al.(2000) noted that D4S1601 and D4S3048, which are located on human chromosome 4p15.3-p15.2, map within intronic sequences of the PROML1 gene. Torrente et al.(2004) reported that a subpopulation of circulating cells expressing AC133, a well-characterized marker of hematopoietic stem cells, also expresses early myogenic markers. Singh et al.(2003) prospectively isolated a CD133-positive cell subpopulation from human brain tumors that exhibited stem cell properties in vitro. However, the true measures of cancer stem cells are their capacity for self renewal and exact recapitulation of the original tumor. |
| 研究类别 | 1. Maw, M. A., Corbeil, D., Koch, J., Hellwig, A., Wilson-Wheeler, J. C., Bridges, R. J., Kumaramanickavel, G., John, S., Nancarrow, D., Roper, K., Weigmann, A., Huttner, W. B., Denton, M. J. A frameshift mutation in prominin (mouse)-like 1 causes human retinal degeneration. Hum. Molec. Genet. 9: 27-34, 2000. 2. Singh, S. K., Clarke, I. D., Terasaki, M., Bonn, V. E., Hawkins, C., Squire, J., Dirks, P. B. Identification of a cancer stem cell in human brain tumors. Cancer Res. 63: 5821-5828, 2003.3. Torrente, Y., Belicchi, M., Sampaolesi, M., Pisati, F., Meregalli, M., D'Antona, G., Tonlorenzi, R., Porretti, L., Gavina, M., Mamchaoui, K., Pellegrino, M. A., Furling, D., Mouly, V., Butler-Browne, G. S., Bottinelli, R., Cossu, G., Bresolin, N. Human circulating AC133(+) stem cells restore dystrophin expression and ameliorate function in dystrophic skeletal muscle. J. Clin. Invest. 114: 182-195, 2004. |
| Uniprot ID | PROM1: O43490 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
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[list_product_images]Figure 1. Western blot analysis of PROM1 using anti-PROM1 antibody (A01767). Lane 1: rat liver tissue lysate,Lane 2: mouse liver tissue lysate,Lane 3: human MCF-7 whole cell lysate,Lane 4: human COLO-320 whole cell lysate,Lane 5: human Hela whole cell lysate. anti-PROM1 antigen affinity purified polyclonal antibody (Catalog # A01767)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for PROM1 at approximately 115-130KD. The expected band size for PROM1 is at 97KD.[/list_product_images]
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Anti-PROM1 Antibody(A01767-50ul)
￥1180
