- ￥1180
- BOSTER
- A01127-50ul
- 中国
- 2025年07月10日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存：
大量
- 供应商：
广州威佳科技有限公司
- 规格：
50ul
产品概况
| 货号 | A01127 |
|---|---|
| 产品名称 | Anti-SFN Antibody |
| 基因名 | SFN |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 28KD |
| 免疫原 | E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248). |
| 内容 | 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500 ug/ml |
| 产品形态 | Liquid |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| 背景资料 | Stratifin(SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium. |
| 研究类别 | 1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E., Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C signalling pathway.J. Molec. Biol. 231: 982-998, 1993.2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets 14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002.3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng, Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to facilitate cytokinesis.Nature 446: 329-332, 2007. |
| Uniprot ID | SFN: P31947 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
产品应用细节
为了提供优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot(WB): | 1:500-2000 |
| Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
| Immunohistochemistry in frozen section: | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow cytometry (FCM): | 1-3 μg/1x106 cells |
| (ELISA)： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片描述
点击图片放大
[list_product_images]Figure 1. Western blot analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody (A01127). Lane 1: human Hela cell lysate,Lane 2: human placenta tissue lysate,Lane 3: human MCF-7 cell lysate,Lane 4: human 22RV1 cell lysate,Lane 5: rat tongue tissue lysate,Lane 6: rat lung tissue lysate,Lane 7: rat PC-12 cell lysate,Lane 8: mouse lung tissue lysate,Lane 9: mouse HEPA1-6 cell lysate,Lane 10: mouse NIH3T3 cell lysate. anti-14-3-3 sigma antigen affinity purified polyclonal antibody (Catalog # A01127)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for 14-3-3 sigma at approximately 28KD. The expected band size for 14-3-3 sigma is at 28KD.|Figure 2. IHC analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody (A01127).
14-3-3 sigma was detected in paraffin-embedded section of human rectal cancer tissues. anti-14-3-3 sigma Antibody (A01127) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody (A01127).
14-3-3 sigma was detected in paraffin-embedded section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody (A01127) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 4. IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody (A01127).
14-3-3 sigma was detected in paraffin-embedded section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody (A01127) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 5. Flow Cytometry analysis of U20S cells using anti- 14-3-3 SIGMA antibody (A01127). Overlay histogram showing U20S cells stained with A01127 (Blue line).anti- 14-3-3 SIGMA Antibody (A01127,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
14-3-3 sigma was detected in paraffin-embedded section of human rectal cancer tissues. anti-14-3-3 sigma Antibody (A01127) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.|Figure 3. IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody (A01127).
14-3-3 sigma was detected in paraffin-embedded section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody (A01127) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 4. IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody (A01127).
14-3-3 sigma was detected in paraffin-embedded section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody (A01127) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.|Figure 5. Flow Cytometry analysis of U20S cells using anti- 14-3-3 SIGMA antibody (A01127). Overlay histogram showing U20S cells stained with A01127 (Blue line).anti- 14-3-3 SIGMA Antibody (A01127,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.[/list_product_images]
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
Anti-14-3-3 sigma/SFN Antibody(A01127-50ul)
￥1180
